MABF155E

Anti-CD44, (human/mouse), PE, clone IM7 Antibody

clone IM7, from rat, PE

• Synonym(s): CD44 antigen, Extracellular matrix receptor III (ECMR-III), GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24 (Ly-24), Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I), CD44
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: PE
• Clone: IM7, monoclonal
• Application: FACS
• Citations: -

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: PE
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: IM7, monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG2bκ
• UniProt accession no.: P15379
• target post-translational modification: unmodified
• Gene Information: human ... CD44(960) ; mouse ... Cd44(12505)

Description

General description

The IM7 antibody recognizes CD44, a ubiquitously expressed cell surface receptor which is important for extracellular matrix organization, cell-cell and cell-matrix adhesion and migration. CD44 may be expressed in a number of different isoforms (splice variants) from the most typical or “standard” form, known as CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands, but most often associate with an extracellular matrix component hyaluronate, through which it mediates adhesion.The IM7 antibody may be used for detection of all isoforms of CD44, as it recognizes constant epitopes near the extracellular proximal domain. (Xu et al, 2002, J. Leukoc. Biol. 72:1133-1141). It has been reported to be cross-reactive with many non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus, Horse, Cow, Pig, Dog and Cat CD44.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-CD44, (human/mouse), PE, clone IM7 Antibody is validated for use in Flow Cytometry for the detection of CD44.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD44 in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg