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MABF167A

Sigma-Aldrich

Anti-CD8 (human), FITC, clone SK1 Antibody

clone SK1, from mouse, FITC conjugate

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Synonym(s):
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

SK1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1, kappa

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CD8A(925)

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This Item
MABF167CMABF168CMABF167B
clone

SK1, monoclonal

clone

SK1, monoclonal

clone

SK1, monoclonal

clone

RPA-T8, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

FITC conjugate

conjugate

PE

conjugate

Peridinin-Chlorophyll-Protein Complex

conjugate

FITC conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

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General description

The SK1 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The SK1 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, RPA-T8, the SK1 antibody will not block binding of RPA-T8 to CD8a.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Anti-CD8 (human), FITC, clone SK1 Antibody is an antibody against CD8 (human), FITC, clone SK1 for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD8 in one million human peripheral blood lymphocytes.

Physical form

Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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