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MABF1983M

Sigma-Aldrich

Anti-CD8a Antibody, clone YTS 169.4

clone YTS 169.4, from rat

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Synonym(s):
T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD_antigen CD8a
eCl@ss:
32160702

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

YTS 169.4, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunodepletion: suitable
immunohistochemistry: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

mouse ... Cd8A(12525)

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MABF153DMABF154CMABF167A
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

YTS 169.4, monoclonal

clone

53-6.7, monoclonal

clone

53-6.7, monoclonal

clone

SK1, monoclonal

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

human

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

packaging

-

technique(s)

flow cytometry: suitable, immunodepletion: suitable, immunohistochemistry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

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General description

T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the Cd8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 is a single-pass type I membrane protein with one Ig-like V-type (immunoglobulin-like) domain. CD8a is produced with a 27 amino acid signal peptide that is subsequently cleaved. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

Specificity

Clone YTS 169.4 detects T-cell surface glycoprotein CD8 alpha chain (CD8a) in mouse thymocytes.

Immunogen

Thymocytes from CBA mouse.

Application

Anti-CD8a, clone YTS 169.4, Cat. No. MABF1983 is a rat monoclonal antibody that detects T-cell surface glycoprotein CD8 alpha chain and has been tested for use in Flow Cytometry, Immunohistochemistry, and Immunodepletion.
Immunodepletion Analysis: A representative lot detected CD8a in Immunodepletion applications (Cobbold, S.P., et. al. (1984). Nature. 312(5994):548-51; Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202; Brown, C.R., et. al. (1990). J Immunol. 145(10):3438-41).

Flow Cytometry Analysis: A representative lot detected CD8a in Flow Cytometry applications (Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202).

Immunohistochemistry Analysis: A representative lot detected CD8a in Immunohistochemistry applications (Wright, J.R. Jr, et. al. (1997). Ann Transplant. 2(3):12-6).
Research Category
Inflammation & Immunology

Quality

Evaluated by Flow Cytometry in mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD8a in mouse thymocytes.

Target description

27.5 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2b in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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