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MABF2067M

Sigma-Aldrich

Anti-Calnexin Antibody, clone AF8

clone AF8, from mouse

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Synonym(s):
IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AF8, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CANX(821)

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AB2301SAB1402138SAB4503258
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

AF8, monoclonal

clone

polyclonal

clone

1D4, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

mouse, rat, human, horse, pig

species reactivity

human

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

capture ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, proximity ligation assay: suitable, western blot: 1-5 μg/mL

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

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General description

Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a well-conserved, calcium binding, endoplasmic reticulum (ER) resident chaperone protein that associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is cleaved to generate the mature form. Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at Ser564 by MAPK3/ERK1, which increases its association with ribosomes.

Specificity

Clone AF8 detects Calnexin in human cells.

Immunogen

TCR complexes isolated from the human T-cell leukemia line, DND41.

Application

Anti-Calnexin, clone AF8, Cat. No. MABF2067, is a mouse monoclonal antibody that detects calnexin in human cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Flow Cytometry Analysis: 1 µg from a representative lot detected Calnexin in one million MCF7 cells.

Immunoprecipitation Analysis: A representative lot detected Calnexin in Immunoprecipitation applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8; Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).

Immunohistochemistry Analysis: A representative lot detected Calnexin in Immunohistochemistry applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).

Flow Cytometry Analysis: A representative lot detected Calnexin in Flow Cytometry applications (Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).

Western Blotting Analysis: A representative lot detected Calnexin in Western Blotting applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Calnexin in HeLa, A431 and Huvec cell lines.
Research Category
Inflammation & Immunology

Quality

Evaluated by Immunocytochemistry in MCF7 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Calnexin in MCF7 cells.

Target description

67.57 kDa calculated.

Linkage

Replaces: MAB3126

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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