MABF2078

Sigma-Aldrich

Anti-APN/CD13 Antibody, clone DF-5

clone DF-5, from mouse

Synonym(s):
EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13
eCl@ss:
32160702
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

DF-5, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

application(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG3κ

NCBI accession no.

UniProt accession no.

Gene Information

human ... ANPEP(290)

Related Categories

General description

Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967).

Specificity

Clone DF-5 specifically detects aminopeptidase N in human cells.

Immunogen

Crude cell membrane fraction from adult human papillary and reticular dermal fibroblasts.

Application

This mouse monoclonal Anti-APN/CD13, clone DF-5, Cat. No. MABF2078, detects Aminopeptidase N and has been tested for use in Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in human prostate tissue.

Flow Cytometry Analysis: A representative lot detected APN/CD13 in Flow Cytometry applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).

Immunofluorescence Analysis: A representative lot detected APN/CD13 in Immunofluorescence applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23; Sorrell, J.M., et. al. (2007). Cell Tissue Res. 327(3):499-510).

Western Blotting Analysis: A representative lot detected APN/CD13 in Western Blotting applications (Sorrell, J.M., et. al. (2003). Exp Dematol. 12(3):315-23).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected APN/CD13 in PC3 cell line.
Research Category
Inflammation & Immunology

Quality

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APN/CD13 in human kidney tissue.

Target description

109.54 kDa calculated.

Physical form

Format: Purified
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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