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MABF2079M

Sigma-Aldrich

Anti-CD8 alpha Antibody, clone 53-6.72

clone 53-6.72, from rat

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Synonym(s):
T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
eCl@ss:
32160702

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

53-6.72, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

mouse ... Cd8A(12525)

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MABF153EMABF153CMABF154B
clone

53-6.72, monoclonal

clone

53-6.7, monoclonal

clone

53-6.7, monoclonal

clone

53-6.7, monoclonal

Gene Information

mouse ... Cd8A(12525)

Gene Information

mouse ... Cd8A(12525)

Gene Information

mouse ... Cd8A(12525)

Gene Information

mouse ... Cd8A(12525)

biological source

rat

biological source

rat

biological source

rat

biological source

rat

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

NCBI accession no.

NP_001074579.1

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

-

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General description

T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets.

Specificity

Clone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes.

Immunogen

Murine Thymic/splenic lymphocytes.

Application

Flow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. al. (2010). Blood. 116(20):4175-84).

Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
This rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies.

Quality

Evaluated by Flow Cytometry in mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.

Target description

27.46 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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