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MABF243

Sigma-Aldrich

Anti-Par-2 Antibody, clone SAM11

clone SAM11, from mouse

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Synonym(s):
Proteinase-activated receptor 2, PAR-2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SAM11, monoclonal

species reactivity

mouse, human

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... F2RL1(2150)

Related Categories

General description

The protein named Proteinase-activated receptor 2 (PAR2/PAR-2), or Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like-1 and encoded by the human gene F2RL1/GPR11/PAR2 is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Also as part of the innate and adaptive immunity response, PAR2 acts as sensor for proteolytic enzymes generated during infection and works to promote inflammation and the inflammatory response. PAR2 is also involved in vascular and airway tone and in blood pressure regulation and many other G protein coupled receptor response pathways. PAR2 is a multi-pass membrane protein that is widely expressed in most tissues except brain or skeletal muscle.

Immunogen

Epitope: N-terminus
Linear peptide corresponding to the N-terminus of human Par-2.

Application

Anti-Par-2 Antibody, clone SAM11 is an antibody against Par-2 for use in western blotting, IHC & flow cytometry.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Par-2 in human small intestine, coronary artery and aorta sections, and in human artery and vein tissues (Molino, M., et al. (1998). Arterioschler Throm Vasc Biol. 18(5):825-832.).

Flow Cytometry Analysis: Arepresentative lot from an independent laboratory detected Par-2 in Par-2 transfected COS-1 cells (Molino, M., et al. (1997). J Biol Chem. 272(17):11133-11141.).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Par-2 in 10 µg of NIH/3T3 cell lysate.

Target description

~50 kDa observed. The calculated MW of this protein is 44 kDa However, it may be observed at higher molecular weights due to glycosylation.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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