MABF328

Sigma-Aldrich

Anti-IL-10 (human) Antibody, PE, clone JES3-19F1

clone JES3-19F1, from rat, PE

Synonym(s):
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

rat

antibody product type

primary antibodies

clone

JES3-19F1, monoclonal

species reactivity

human

application(s)

flow cytometry: suitable

isotype

IgG2aκ

conjugate

PE

UniProt accession no.

shipped in

wet ice

Gene Information

human ... IL10(3586)

General description

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) is a protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. IL-10 down regulates the expression of Th1 cytokines, major histocompatibility complex class II antigens, and costimulatory molecules on macrophages. It also enhaces B cell survival, proliferation, and antibody production. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. IL-10 is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. A polymorphism in IL10 promoter region is associated with resistance to graft-versus-host disease (GVHDS), a major complication of allogeneic bone marrow transplantation, in which mature donor T-cells that contaminate the allogeneic bone marrow recognize the tissues of the recipient as foreign, causing a severe inflammatory disease. Knockout studies suggest that IL-10 is an immunoregulator in the intestinal tract.

Immunogen

COS-expressed recombinant human IL-10

Application

Research Sub Category
Cytokines & Cytokine Receptors
Anti-IL-10 (human) Antibody, PE, clone JES3-19F1 is a highly specific rat monoclonal antibody & that targets IL-10 & has been tested in Flow Cytometry.
Research Category
Inflammation & Immunology

Quality

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Flow Cytometry Analysis: 20 μL of this antibody detected IL-10 in one million LPS-stimulated human peripheral blood monocytes.

Target description

21 kDa calculated

Physical form

Affinity
Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.

Storage and Stability

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.