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MABF472

Sigma-Aldrich

Anti-Ly-6G (Gr-1) Antibody (mouse), PE, clone RB6-8C5

clone RB6-8C5, 0.2 mg/mL, from rat

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Synonym(s):
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

PE

antibody form

purified antibody

antibody product type

primary antibodies

clone

RB6-8C5, monoclonal

species reactivity

mouse

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ly6G(546644)

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MABF473MABF474MABF147E
Gene Information

mouse ... Ly6G(546644)

Gene Information

mouse ... Ly6G(546644)

Gene Information

mouse ... Ly6G(546644)

Gene Information

mouse ... Ly6G(546644)

clone

RB6-8C5, monoclonal

clone

RB6-8C5, monoclonal

clone

RB6-8C5, monoclonal

clone

RB6-8C5, monoclonal

conjugate

PE

conjugate

PE-Cy7

conjugate

-

conjugate

-

biological source

rat

biological source

rat

biological source

rat

biological source

rat

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

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General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports sµggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This rat monoclonal antibody Anti-Ly-6G (Gr-1) (mouse), PE, clone RB6-8C5 validated for use in IHC & Flow Cytometry for the detection of Ly-6G (Gr-1).

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected Ly-6G (Gr-1) in one million C57Bl/6 bone marrow cells.

Physical form

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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