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MABF557

Sigma-Aldrich

Anti-CD28 Antibody (human), APC, clone CD28.2

clone CD28.2, from mouse

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Synonym(s):
T-cell-specific surface glycoprotein CD28, TP44, CD antigen CD28
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

Allophycocyanin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

CD28.2, monoclonal

species reactivity

human

packaging

pkg of 5 μL (0.25 μg/test)

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD28(940)

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This Item
MABF409MABF559MABF411
conjugate

Allophycocyanin conjugate

conjugate

PE

conjugate

PE-Cy7

conjugate

FITC conjugate

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

CD28.2, monoclonal

clone

CD28.2, monoclonal

clone

CD28.2, monoclonal

clone

CD28.2, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

packaging

pkg of 5 μL (0.25 μg/test)

packaging

pkg of 5 μL (0.25 μg/test)

packaging

pkg of 5 μL (0.25 μg/test)

packaging

pkg of 5 μL (0.5 μg/test)

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General description

The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo. This antibody is also reported to be cross-reactive with several non-human species, including Baboon, Chimpanzee, Cynomolgus, and Rhesus.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD28 (human), APC & clone CD28.2 validated for use in IP;IHC;Flow Cytometry for the detection of CD28.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD28 in one million human peripheral blood lymphocytes.

Physical form

Purified mouse monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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