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MABF81

Sigma-Aldrich

Anti-ROR gamma T Antibody, clone 6F3.1

clone 6F3.1, from mouse

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Synonym(s):
Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6F3.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RORC (6097)

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This Item
SAB4502650AV32256MABS496
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

6F3.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

6G1, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

rat, mouse

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG2aκ

isotype

-

isotype

-

isotype

IgG1

General description

Retinoid-related orphan receptor-gamma t (RORγT) is one of two RORγ isoforms belonging to the nuclear hormone receptor family and contains a single nuclear receptor DNA-binding domain. RORγT is a negative regulator of TNFDF6/FASL expression and T-cell production of IL2. The upregulation of RORγT is a requirement for TH17 cell differentiation. Inhibition of RORγT is induced by TGF-β’s interaction with Foxp3. Expression is observed mainly in immature thymocytes, and, during fetal development, is found exclusively in lymphoid-inducer (LTi) cells.

Immunogen

GST-tagged recombinant protein corresponding to human ROR gamma T.

Application

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ROR gamma T in normal human liver tissues.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-ROR gamma T Antibody, clone 6F3.1 is validated for use in WB, IH for the detection of ROR gamma T.

Quality

Evaluted in Western Blot in T47D cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected ROR gamma T in 10 µg of T47D cell lysate.

Target description

~55 kDa observed. Two isoforms at ~55 kDa and ~58 kDa may be observed in some cell lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
T47D cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Huai-Chia Chuang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(10), 11469-11480 (2019-07-19)
The cytokine IL-17A plays critical roles in the pathogenesis of autoimmune diseases. The frequencies of MAP kinase kinase kinase kinase 3 [also named germinal center kinase-like kinase (GLK)]-overexpressing T cells are correlated with disease severity of systemic lupus erythematosus (SLE).
Wei-Bin Shen et al.
Gynecologic and obstetric investigation, 87(2), 165-172 (2022-05-09)
Studies indicate a very low rate of SARS-CoV-2 detection in the placenta or occasionally a low rate of vertical transmission in COVID-19 pregnancy. SARS-CoV-2 Delta variant has become a dominant strain over the world and possesses higher infectivity due to
Atsuyo Ikeda et al.
Cancer immunology research, 8(6), 724-731 (2020-04-02)
Innate lymphoid cells (ILC) are responsible for mucosal tissue homeostasis and are involved in the progression and suppression of several types of cancer. However, the effects of ILCs on colorectal cancer are poorly understood. We characterized human ILCs in normal
Tae Gyu Oh et al.
EBioMedicine, 6, 59-72 (2016-05-24)
We have previously reported that RORγ expression was decreased in ER-ve breast cancer, and increased expression improves clinical outcomes. However, the underlying RORγ dependent mechanisms that repress breast carcinogenesis have not been elucidated. Here we report that RORγ negatively regulates
Hiroki Kanno et al.
Cancer science, 111(8), 3032-3044 (2020-05-26)
Tumor location and immunity play important roles in the progression of colorectal cancer (CRC). This study aimed to investigate the differences in the immunosurveillance pattern between right- and left-sided CRC and analyze their association with clinicopathologic features, including mismatch repair

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