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MABN1190

Sigma-Aldrich

Anti-PSD95 Antibody, clone 1A8.1

clone 1A8.1, from mouse

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Synonym(s):
Disks large homolog 4, Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A8.1, monoclonal

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... DLG4(1742)

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This Item
ABN998MABN68P246
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

1A8.1, monoclonal

clone

polyclonal

clone

K28/43, monoclonal

clone

7E3-1B8, monoclonal

species reactivity

rat, human

species reactivity

human

species reactivity

rat

species reactivity

rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), inhibition assay: suitable (peptide), western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: 1:500, immunoprecipitation (IP): suitable, western blot: 1:2,000

isotype

IgG1κ

isotype

-

isotype

IgG2aκ

isotype

IgG2a

General description

Disks large homolog 4 (UniProt P78352; also known as Postsynaptic density protein 95, PSD-95, SAP-90, SAP90, Synapse-associated protein 90) is encoded by the DLG4 (also known as PSD95) gene (Gene ID 1742) in human. PSD-95 belong to the family of membrane-associated guanylate kinases (MAGUKs), which also include PSD-93, SAP97 and SAP102. PSD95 is predominantly expressed in the hippocampus CA1 region and prefrontal cortex of the brain, where it localizes in a somatodendritic pattern in the post-synaptic membrane and in presynaptic axon terminals of inhibitory neurons. PDS-95 is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength via interactions with a diverse PSD proteins, including NMDA receptors (NMDARs), AMPA receptor (AMPAR) complexes via Stargazin/TARP, adhesion molecules, as well as other scaffolding proteins, such as GKAP and Shank. PSD95 and neuronal nitric-oxide synthase (nNOS) interaction plays a role in maintaining hypersensitivity in acute and chronic pain. In addition, the assembly of the GluR6-PSD95-CaMKII complex is implicated as a majore signaling module that mediates injury induced by brain ischemia by facilitating GluR6 serine phosphorylation by CaMKII and the activation of JNK.

Specificity

Clone 1A8.1 epitope sequence is present in all spliced isoforms of human, mouse, and rat PSD95 reported by UniProt (P78352, Q62108, P31016).

Immunogen

Epitope: Region between PDZ domain 2 & 3.
KLH-conjugated linear peptide corresponding to the region between PDZ domain 2 & 3 of human PSD95.

Application

Anti-PSD95 Antibody, clone 1A8.1 is an antibody against PSD95 for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot PSD95 in human cerebral cortex and cerebellum tissue sections.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Quality

Evaluated by Western Blotting in rat hippocampus tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSD95 in 10 µg of rat hippocampus tissue lysate.

Target description

~80 kDa observed. 80.50/80.47/80.47 kDa human/mouse/rat isoform 1; PSD95-alpha), 85.43/85.42 kDa (human/mouse isoform 2; PSD95-beta), 80.13/80.10 kDa (human/mouse isoform 3) calculated.

Physical form

Format: Purified
Protein G purified.
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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