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MABN1618

Sigma-Aldrich

Anti-Nicotinic Acetylchone Receptor alpha 1 Antibody, clone 7C8.2

clone 7C8.2, from mouse

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Synonym(s):
Acetylcholine receptor subunit alpha, AChR nicotinic alpha 1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7C8.2, monoclonal

species reactivity

rat, human

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CHRNA1(1134)

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This Item
SAB1402724AV34909MABN2249
clone

7C8.2, monoclonal

clone

2G5, monoclonal

clone

polyclonal

clone

5B2.1, monoclonal

Gene Information

human ... CHRNA1(1134)

Gene Information

human ... CHRNA1(1134)

Gene Information

human ... CHRNA1(1134)

Gene Information

human ... CHRNA2(1135)

species reactivity

rat, human

species reactivity

human

species reactivity

human

species reactivity

human

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

NCBI accession no.

NP_001034612

NCBI accession no.

NM_000079

NCBI accession no.

NP_000070

NCBI accession no.

NP_000733

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General description

Acetylcholine receptor subunit alpha (UniProt: P02708; AChR nicotinic alpha 1) is encoded by the CHRNA1 (also known as ACHRA, CHNRA) gene (Gene ID: 1134) in human. Nicotinic Acetylcholine Receptor is a member of the ligan-gated ion channel family. It is a multi-pass membrane protein on the post-synaptic cell membrane. Nicotinic Ach receptor is a pentamer of two alpha chains and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains. Two isoforms of this receptor have been described that are produced by alternative splicing. Isoform 1 is only expressed in skeletal muscle and isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus. It contains an extracellular domain (aa 21-255), four helical domains (aa 256-280; 288-306; 322-341; and 454-472) and a cytoplasmic domain (aa 342-453). After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA1 gene are linked to Myasthenic syndrome, which is characterized by muscle weakness affecting the axial and limb muscles, and the ocular muscles that lead to ptosis and opthalmoplegia. The alpha subunit is the main focus for antibody binding in myasthenia gravis.

Specificity

Clone 7C8.2 detects nicotinic acetylcholine receptor alpha in human and rat. It targets an epitope within 97 amino acids from the C-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 97 amino acids from the C-terminal half of human nicotinic acetylcholine receptor alpha.

Application

Anti-Nicotinic Acetylchone Receptor alpha 1, clone 7C8.2, Cat. No. MABN1618, is a highly specific mouse monoclonal antibody that targets CHRNA1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Nicotinic Acetylchone Receptor alpha 1 in 10 µg of SW480 cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Nicotinic Acetylchone Receptor alpha 1 in human colon cancer tissue sections.

Quality

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nicotinic Acetylchone Receptor alpha 1 in 10 µg of L6 cell lysate.

Target description

~55 kD observed; 54.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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