MilliporeSigma
All Photos(3)

Documents

MABN1724

Sigma-Aldrich

Anti-GABA(A) Receptor α2 Antibody, clone N399/19

clone N399/19, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2, Gamma-aminobutyric acid receptor A2 subunit
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N399/19, monoclonal

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GABRA2 (2555)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
G0295MABN263MABN875
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

rat, human, mouse

species reactivity

rat

species reactivity

rat

species reactivity

rat, bovine, human, mouse

clone

N399/19, monoclonal

clone

polyclonal

clone

10F10-C1-B8, monoclonal

clone

KC4-8A7, monoclonal

Gene Information

human ... GABRA2 (2555)

Gene Information

rat ... Gabra6(29708)

Gene Information

rat ... Gabrg2(29709)

Gene Information

human ... GABRG2(2566)

isotype

IgG1κ

isotype

-

isotype

IgG2bκ

isotype

IgG2aκ

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Gamma-aminobutyric acid receptor subunit alpha-2 (UniProt P23576; also known as GABA(A) receptor subunit alpha-2, Gamma-aminobutyric acid receptor A2 subunit) is encoded by the Gabra2 (also known as Gabra-2) gene (Gene ID 289606) in rat species. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Its endogenous ligand is the inhibitory neurotransmitter γ-aminobutyric acid (GABA) in the central nervous system (CNS). Upon activation, the GABAA receptor selectively conducts chloride ion through its pore, resulting in hyperpolarization of the neuron. The receptor is a pentameric complex composed of two alpha, two beta, and one gamma subunits arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human and rat, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The alpha 2 subunit is intially produced with a signal peptide sequence (aa 1-28), which is then cleaved off to yield the mature subunit (aa 29-451) with a large N-terminal extracellular domain (a.a. 29-251), four transmembrane segments (a.a. 252-273, 279-300, 313-334, 420-441) and a large intracellular/cytoplasmic loop (a.a. 335-419) between the third and fourth transmembrane domain.

Specificity

Clone N399/19 detects the GABA(A) receptor subunit alpha-2 band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice. Clone N399/19 does not cross-react with GABA(A) receptor subunit alpha-1.

Immunogen

Epitope: Cytoplasmic domain.
Recombinant rat GABA(A) receptor α2 cytoplasmic fragment.

Application

Detect GABA(A) Receptor α2 using this Anti-GABA(A) Receptor α2 Antibody, clone N399/19 validated for use in Western Blotting, Immunohistochemistry.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABA(A) receptor subunit alpha-2 in 10 µg rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected the GABA(A) receptor subunit alpha-2 target band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice.
Immunohistochemisry Analysis: A representative lot detected GABA(A) receptor subunit alpha-2 immunoreactive regions, includinghippocampus, in rat whole brain sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABA(A) receptor subunit alpha-2 in 10 µg human brain tissue lysate.

Target description

~57 kDa observed. Target band appears larger than the calculated molecular weight of 47.94 kDa due to glycosylation.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Pradeep Kannampalli et al.
Neuroscience, 346, 349-364 (2017-01-28)
Painful events early in life have been shown to increase the incidence of interstitial cystitis/painful bladder syndrome in adulthood. However, the intrinsic mechanism is not well studied. We previously reported that neonatal bladder inflammation causes chronic visceral hypersensitivity along with
Gloria S Forkuo et al.
Molecular pharmaceutics, 13(6), 2026-2038 (2016-04-28)
Recent studies have demonstrated that subtype-selective GABAA receptor modulators are able to relax precontracted human airway smooth muscle ex vivo and reduce airway hyper-responsiveness in mice upon aerosol administration. Our goal in this study was to investigate systemic administration of
Gloria S Forkuo et al.
Molecular pharmaceutics, 14(6), 2088-2098 (2017-04-26)
We describe pharmacokinetic and pharmacodynamic properties of two novel oral drug candidates for asthma. Phenolic α4β3γ2 GABAAR selective compound 1 and acidic α5β3γ2 selective GABAAR positive allosteric modulator compound 2 relaxed airway smooth muscle ex vivo and attenuated airway hyperresponsiveness

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service