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MABN1775

Sigma-Aldrich

Anti-ABCA4 Antibody, clone TMR1

clone TMR1, from mouse

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Synonym(s):
Retinal-specific ATP-binding cassette transporter, ATP-binding cassette sub-family A member 4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

TMR1, monoclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

bovine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ABCA4(24)

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MABN287ABN1441MABN893
Anti-ABCA4 Antibody, clone TMR1 clone TMR1, from mouse

MABN1775

Anti-ABCA4 Antibody, clone TMR1

Anti-ABCA4 Antibody, clone 5D7.1 clone 5D7.1, from mouse

MABN287

Anti-ABCA4 Antibody, clone 5D7.1

Anti-ABCA4 from rabbit, purified by affinity chromatography

ABN1441

Anti-ABCA4

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rat

species reactivity

human, mouse

species reactivity

human

species reactivity

human

species reactivity

mouse, human

Gene Information

human ... ABCA4(24)

Gene Information

human ... ABCA4(24)

Gene Information

human ... ABCA4(24)

Gene Information

bovine ... Abca1(535379)

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunofluorescence: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

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General description

Retinal-specific ATP-binding cassette transporter (UniProt F1MWM0/P78363; also known as ATP-binding cassette sub-family A member 4, ABC transporter A4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein) is encoded by the ABCA4 (also known as ABCR) gene (Gene ID 281584/24) in bovine/human species. ABC transporter A4, a ~250 kDa single-chain, is a multi-pass membrane protein of the superfamily of ATP-binding cassette (ABC) transporters that is localized to the disk margins of vertebrate photoreceptor outer segments. It is implicated in the clearance of all-trans retinal derivatives from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. ABC transporter A4 is composed of two symmetrically organized halves, each comprising six membrane-spanning helices, a large glycosylated exocytoplasmic domain located inside the disk, and a cytoplasmic domain with an ATP-binding cassette. Mutations in ABCA4 are known to cause Stargardt macular degeneration, which is an autosomal recessive form of juvenile macular degeneration associated with a severe loss in vision.

Ref.: Tsybovsky, Y et al. (2010). Adv. Exp. Med. Biol. 703, 105-125.

Specificity

Clone TMR1 immunostained retina cryosections from wild-type mice, but not retina from mice with homozygous L541P;A1038V (PV) missense mutation (Courtesy of Grace Jin, Case Western University, U.S.A.).

Immunogen

ABCA4 isolated from bovine retinas (Zhang, N., et al. (2015). Hum. Mol. Genet. 24(11):3220-3237).

Application

Detect ABCA4 using this mouse monoclonal Anti-ABCA4 Antibody, clone TMR1, Cat. No. MABN1775, validated for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Immunofluorescence Analysis: A 1:25-125 dilution from a representative lot detected ABCA4 immunoreactivity in retina cryosections from wild-type mice, but not in retina from mice with homozygous L541P;A1038V (PV) missense mutation (Courtesy of Grace Jin, Case Western University, U.S.A.).

Immunocytochemistry Analysis: A representative lot detected similar cellular localization of wild-type and ABCA4 missense constructs with either or both L541P and A1038V mutation by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 transfectants (Zhang, N., et al. (2015). Hum. Mol. Genet. 24(11):3220-3237).

Immunofluorescence Analysis: A representative lot detected ABCA4 immunoreactivity in retina cryosections from wild-type mice and mice with heterozygous L541P;A1038V (PV) missense mutaion, but not mice with homozygous PV mutation (Zhang, N., et al. (2015). Hum. Mol. Genet. 24(11):3220-3237).
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected ABCA4 in 10 µg of human retina tissue lysate.


Target description

~257 kDa observed. 257.4/255.9/260.2 kDa (bovine/human/mouse) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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