Dual specificity tyrosine-phosphorylation-regulated kinase 1A (EC 184.108.40.206; UniProt Q63470; also known as Dual specificity YAK1-related kinase, MNBH, Protein kinase minibrain homolog, RP86) is encoded by the Dyrk1a (also known as Dyrk) gene (Gene ID 25255) in rat species. DYRK1A belongs to a family of conserved dual-specificity tyrosine-phosphorylated and regulated kinases (DYRKs) within the CMGC (CDK, MAPK, GSK, and CLK) group of eukaryote kinome. DYRK family members share a conserved kinase domain and an adjacent DYRK-homology domain or DH-box (DDDNXDY), wihile differing in their N- and C-terminal regions. DYRK1A is regulated by autophosphorylation on Ser, Thr and Tyr residues, but catalyzes phosphorylation of a broad-range of substrates only on Ser/Thr residues within the RPX(S/T)P motif with a preference for Pro at the +1 position (X). Known DYRK1A substrates include transcription factors (e.g. FKHR, CREB, Gli1, NFAT), splicing factors (e.g. cycling L2, SF3b/SAP155), glycogen synthase, as well as multiple proteins engaged in endocytosis in neurons, including dynamin and synaptojanin 1. DYRK1A is also implicated in promoting the formation of neurotoxic Aβ peptides by phosphorylating APP and presenilin-1. Human DYRK1A gene is located in the Down syndrome critical region of chromosome 21 and the protein plays an essential role in the development of the central nervous system. DYRK1A haploinsufficiency and mutations are the causes of intellectual disability (ID), microcephaly and dysmorphic features in human, while transgenic mice carrying extra copies of the gene exhibit learning defects and motor abnormalities.
The specificity of target band detection was confirmed by antibody blocking with an excess amount of DYRK1A prior to Western blotting (Murakami, N., et al. (2009). Biochemistry. 48(39):9297-9305).
His-tagged recombinant rat DYRK1A N-terminal fragment.
Anti-DYRK1A, clone 8D9, Cat. No. MABN1848, is a mouse monoclonal antibody that targets DYRK1A isoforms and has been tested in Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected nuclear DYRK1A immunoreactivity in human (prostate and pancreatic) cancer tissue and rat cerebral cortex tissue sections.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected DYRK1A in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A representative lot detected DYRK1A in NIH/3T3 cell lysate and human frontal cortex homogenates, as well as in DYRK1A and actin immunoprecipitates (Dowjat, K., et al. (2012). J. Neuropathol. Exp. Neurol. 71(12):1100-1112).
Western Blotting Analysis: A representative lot detected DYRK1A levels in lymphoblastoid cell lines (LCLs) from healthy control, Down syndrome (DS) and fragile X (FraX) human subjects (Dowjat, K., et al. (2012). J. Neuropathol. Exp. Neurol. 71(12):1100-1112).
Western Blotting Analysis: Representative lots detected DYRK1A in rat brain tissue homogenate and subfractionated extracts. The nuclear fraction had low DYRK1A, whereas the majority DYRK1A was found in the postnuclear fractions with clathrin-coated vesicles (CCVs) containing the highest level (Murakami, N., et al. (2012). PLoS One. 7(4):e34845; Murakami, N., et al. (2009). Biochemistry. 48(39):9297-9305).
Western Blotting Analysis: A representative lot detected recombinant rat DYRK1A-bound protein bands by a Western blotting-based DYRK1A overlay assay (Murakami, N., et al. (2009). Biochemistry. 48(39):9297-9305).
Western Blotting Analysis: A representative lot detected recombinant rat DYRK1A in GST-End1, but not GST-End2, pull-down. Clone 8D9 detected the C-terminal PEST domain deletion DYRK1A497 construct in the GST-amphiphysin SH3 domain (GST-Am-SH3) pull-down only in the presence of dynamin (Murakami, N., et al. (2009). Biochemistry. 48(39):9297-9305).
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DYRK1A in 10 µg of HEK293 cell lysate.
~90 kDa observed. 85.58/84.56/60.33/61.77/66.10 kDa (human isoform Long/1/2/3/4), 85.49 kDa (mouse), 85.54/84.51 kDa (rat isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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