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MABN1871

Sigma-Aldrich

Anti-HCN4 Antibody, clone PG2-1A4

clone PG2-1A4, from rat

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Synonym(s):
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, Brain cyclic nucleotide-gated channel 3, BCNG-3
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PG2-1A4, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μL

technique(s)

electron microscopy: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

mouse ... Hcn4(330953)

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antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

clone

PG2-1A4, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse

species reactivity

rat, mouse, human

species reactivity

rat

species reactivity

rat

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

packaging

-

technique(s)

electron microscopy: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

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General description

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (UniProt: O70507; also known as Brain cyclic nucleotide-gated channel 3, BCNG-3, HCN4) is encoded by the Hcn4 (also known as Bcng3) gene in murine species. HCN4 is a homo- or hetero-tetrameric multi-pass membrane protein that is detected in a subset of elongated cells in taste buds. It acts as a hyperpolarization-activated ion channel with very slow activation and inactivation. It exhibits weak selectivity for potassium over sodium ions. HCN4 is reported to contribute to the native pacemaker currents in heart that regulates the rhythm of heart beat. It may also contribute to the native pacemaker currents in neurons. HCN4 is activated by cAMP and cAMP binding causes a conformation change that leads to the assembly of an active tetramer and induces channel opening. This channel may mediate responses to sour stimuli.

Specificity

Clone PG2-1A4 detects murine Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4). It targets an epitope within 70 amino acids from the C-terminal region.

Immunogen

GST-tagged recombinant fragment corresponding to 70 amino acids from the C-terminal region of murine Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4).

Application

Anti-HCN4, clone PG2-1A4 Antibody, Cat. No. MABN1871, is a highly specific rat monoclonal antibody that targets murine HCN4 and has been tested for use in immunofluorescence, Immunohistochemistry, Electron Microscopy, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected HCN4 in Immunofluorescence applications (Cichy, A., et. al. (2015). J Neurosci. 35(9):4025-39).

Immunohistochemistry Analysis: A 1:100-500 dilution from a representative lot detected HCN4 in Mouse (C57BL/6) retina tissues (Courtesy of Prof. Dr. Frank Müller, Institute of Complex Systems, Cellular Biophysics (ICS-4), Forschungszentrum Jülich, Germany).

Electron Microscopy Analysis: A representative lot detected HCN4 in Electron Microscopy applications (Mataruga, A., et. al. (2007). J Comp Neurol. 502(6):1123-37).

Immunohistochemistry Analysis: A representative lot detected HCN4 in Immunohistochemistry applications (Cichy, A., et. al. (2015). J Neurosci. 35(9):4025-39; Mataruga, A., et. al. (2007). J Comp Neurol. 502(6):1123-37).

Western Blotting Analysis: A representative lot detected HCN4 in Western Blotting applications (Muller, F., et. al. (2003). Eur J Neruosci. 17(10):2084-96).

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.

Target description

127.41 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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