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MABN2249

Sigma-Aldrich

Anti-CHRNA2 Antibody, clone 5B2.1

clone 5B2.1, from mouse

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Synonym(s):
Neuronal acetylcholine receptor subunit alpha-2, Cholinergic Receptor Nicotinic Alpha 2 Subunit, Acetylcholine Receptor Nicotinic Alpha 2
eCl@ss:
32160702

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5B2.1, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CHRNA2(1135)

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This Item
AV34909WH0001138M1AV37669
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

Gene Information

human ... CHRNA2(1135)

Gene Information

human ... CHRNA1(1134)

Gene Information

human ... CHRNA5(1138)

Gene Information

human ... CHRNB2(1141)

clone

5B2.1, monoclonal

clone

polyclonal

clone

7D3, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

pig, human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

western blot: suitable

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General description

Neuronal acetylcholine receptor subunit alpha-2 (UniProt: Q15822; also known as Cholinergic Receptor Nicotinic Alpha 2 Subunit, Acetylcholine Nicotinic Receptor Alpha 2) is encoded by the CHRNA2 gene (Gene ID: 1135) in human. Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. In neuronal AChR alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Cholinergic Receptor Nicotinic Alpha 2 Subunit is a multi-pass membrane protein that is produced with a signal peptide (aa 1-26), which is subsequently cleaved off. It has an extracellular domain (aa 27-264), a cytoplasmic domain (aa 353-502), and four helical transmembrane domains (aa 265-289; 297-315; 331-352; 503-521). Mutations in CHRNA2 gene are known to cause epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering.

Specificity

Clone 5B2.1 detects human Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2). It targets an epitope within 52 amino acids from the N-terminal region extracellular domain.

Immunogen

Epitope: extracellular domain
GST/His-tagged recombinant fragment corresponding to 52 amino acids from the N-terminal region of human Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2).

Application

Anti-CHRNA2, clone 5B2.1 Antibody, Cat. No. MABN2249, is a highly specific mouse monoclonal antibody that targets Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHRNA2 in human pancreas, tonsil, and skeletal muscle tissue sections.
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in CHRNA2 recombinant protein lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected human recombinant Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2).

Target description

59.77 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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