MilliporeSigma
All Photos(1)

Documents

MABN2440

Sigma-Aldrich

Anti-ABCA4 Antibody, clone 5B4

clone 5B4, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Retinal-specific ATP-binding cassette transporter, ATP-binding cassette sub-family A member 4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5B4, monoclonal

species reactivity

bovine, human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ABCA4(24)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABN287MABN1775MABN2439M
Anti-ABCA4 Antibody, clone 5B4 clone 5B4, from mouse

MABN2440

Anti-ABCA4 Antibody, clone 5B4

Anti-ABCA4 Antibody, clone 5D7.1 clone 5D7.1, from mouse

MABN287

Anti-ABCA4 Antibody, clone 5D7.1

Anti-ABCA4 Antibody, clone TMR1 clone TMR1, from mouse

MABN1775

Anti-ABCA4 Antibody, clone TMR1

Anti-ABCA4 Antibody, clone 3F4 clone 3F4, from mouse

MABN2439M

Anti-ABCA4 Antibody, clone 3F4

Gene Information

human ... ABCA4(24)

Gene Information

human ... ABCA4(24)

Gene Information

human ... ABCA4(24)

Gene Information

human ... ABCA4(24)

species reactivity

bovine, human

species reactivity

human

species reactivity

human, mouse

species reactivity

bovine, human, mouse

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

5B4, monoclonal

clone

5D7.1, monoclonal

clone

TMR1, monoclonal

clone

3F4, monoclonal

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

shipped in

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Retinal-specific ATP-binding cassette transporter (UniProt: P78363; also known as ATP-binding cassette sub-family A member 4, ABCA4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein) is encoded by the ABCA4 (also known as ABCR) gene (Gene ID: 24) in human. ABCA4 is a multi-pass membrane protein that is exclusively expressed in retina and is found in the rims of rod photoreceptor cells. It contains two ABC transporter domains (aa 929-1160 and 1938-2170). In the visual cycle, it act as a retinal extruder or retinal-phosphatidylethanolamine flippase to facilitate the removal of all-trans-retinal from disc membranes following the photobleaching of rhodopsin. Mutations in ABCA4 gene are reported to cause Stargardt disease, which is characterized by decreased central vision, atrophy of the macula and underlying retinal pigment epithelium, and frequent presence of prominent flecks in the posterior pole of the retina. It has also been linked to age-related macular degeneration and retinitis pigmentosa.

Specificity

Clone 5B4 detects Retinal-specific ATP-binding cassette transporter protein in human and bovine. It targets an epitope within 249 amino acids from the N-terminal half.

Immunogen

GST-tagged recombinant fragment corresponding to 249 amino acids from the N-terminal half of human Retinal-specific ATP-binding cassette transporter.

Application

Anti-ABCA4, clone 5B4, Cat. No. MABN2440, is a highly specific mouse monoclonal antibody that targets Retinal-specific ATP-binding cassette transporter and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected ABCA4 in Immunocytochemistry applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of human retina tissue lysate.

Target description

250 kDa observed; 255.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

T Cronin et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e21934-e21934 (2021-10-03)
Dysfunction of the ATPase-binding Cassette Transporter protein (ABCA4) can lead to early onset macular degeneration, in particular to Stargardt disease. To enable translational research into this form of blindness, we evaluated the effect of Cas9-induced disruptions of the ABCA4 gene

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service