Anti-Cdc42 Antibody, clone 28-10

clone 28-10, from mouse

Cell division control protein 42, Suppressor of RHO3 protein 2

biological source


antibody product type

primary antibodies


28-10, monoclonal

species reactivity

mouse, human, yeast


antibody small pack of 25 μL


immunocytochemistry: suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

Gene Information

human ... CDC42(998)

Related Categories

General description

Cell division control protein 42 (UniProt: P19073; also known as Cdc42, Suppressor of RHO3 protein 2) is encoded by the CDC42 (also known as SRO2, TLR229C, L8083.13) gene (Gene ID: 850930) in yeast (Saccharomyces cerevisiae). Cdc42 is a member of the Rho GTPase family that regulates multiple signaling pathways involved in actomyosin organization, cell proliferation, and maintenance of cell polarity in most eukaryotic cell types. Cdc42 signaling process involves guanine nucleotide exchange factors (GEFs), guanine nucleotide dissociation inhibitors (GDIs), and GTPase-activating proteins (GAPs). In yeast, generation and maintenance of robust Cdc42 polarity promotes membrane expansion during bud formation and it has been shown that Cdc42 is dynamically maintained at the polarity cap through its continuous cycling between the polarity cap and internal pools. Cdc42 is highly polarized during bud emergence and is specifically required for exocytosis at this stage. Cdc42 is synthesized with a propeptide region (aa 189-191), which is cleaved off in the mature form. The mature form contains three nucleotide (GTP)-binding sites. (Ref.: Wu, H., and Brennwald, P. (2010) Mol. Cell Biol. 30(21); 5207-5217).


Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).


GST-tagged full-length recombinant yeast Cdc42.


Anti-Cdc42, clone 28-10, Cat. No. MABN2485, is a highly specific mouse monoclonal antibody that targets Cell division control protein 42 (Cdc42) and has been tested for use in Immunocytochemistry and Western Blotting.
Research Category
Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).

Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).


Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc42 in HeLa cell lysate.

Target description

~21 kDa observed; 21.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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