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MABN328

Sigma-Aldrich

Anti-peptidyl-citrulline, clone F95 Antibody

clone F95, from mouse

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Synonym(s):
Peptidyl-citrulline, Protein-bound citrulline
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

F95, monoclonal

species reactivity

mouse, rat, chicken, human

species reactivity (predicted by homology)

all (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

shipped in

wet ice

target post-translational modification

unmodified

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This Item
MABS487MABT1488HPA017007
antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

F95, monoclonal

clone

C4, monoclonal

clone

3C6, monoclonal

clone

polyclonal

species reactivity

mouse, rat, chicken, human

species reactivity

-

species reactivity

mouse, human

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200

isotype

IgMκ

isotype

IgG1λ

isotype

IgG1κ

isotype

-

General description

Deimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.

Immunogen

Citrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.

Quality

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.

Target description

Various kDa calculated

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Robby Engelmann et al.
PloS one, 11(6), e0158773-e0158773 (2016-07-01)
To assess the prerequisites for negative selection of peptidylcitrulline-specific T cells in the thymus. In detail, we here analyzed murine medullary thymic epithelial cells for the expression of peptidylarginine deiminases (PAD) and subsequent citrullination. Medullary thymic epithelial cells were sorted
Marianne Engström et al.
Journal of translational medicine, 16(1), 214-214 (2018-08-02)
A relationship between rheumatoid arthritis (RA) and periodontitis has been suggested from findings that individuals with RA are prone to have advanced periodontitis and vice versa. In search of possible common pathogenetic features of these two diseases, we investigated the
R Bright et al.
Journal of periodontal research, 53(1), 139-143 (2017-10-19)
Postranslational modification of proteins can lead to the production of autoantibodies and loss of immune tolerance. This process has been hypothesised to be a critical factor in the pathogenesis of rheumatoid arthritis. The objective of this study was to demonstrate
Caitlyn L Holmes et al.
PloS one, 14(5), e0217221-e0217221 (2019-05-29)
The antibody response to pertussis vaccination in rheumatoid arthritis is unknown, a concerning omission given the relatively low efficacy of the pertussis vaccine, a rise in pertussis infections, and a general increased susceptibility to infection in rheumatoid arthritis. Additionally, the
Michael F Criscitiello et al.
International journal of molecular sciences, 21(8) (2020-04-25)
The bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. PADs also regulate extracellular vesicle

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