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MABN464

Sigma-Aldrich

Anti-Cav3.1 Ca2+ channel Antibody, clone N178A/9

clone N178A/9, from mouse

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Synonym(s):
Voltage-gated calcium channel, alpha-1-G subunit
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N178A/9, monoclonal

species reactivity

mouse, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Cacna1G(12291)

General description

Voltage-dependent T-type calcium channel subunit alpha-1G or Voltage-gated calcium channel subunit alpha Cav3.1, like other members of the voltage-sensitive calcium channel (VSCC) family, mediate the entry of calcium ions into excitable cells. These channels regulate and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1G gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and also support calcium signaling in secretory cells and vascular smooth muscle.

Specificity

Other homologies: Human (80% sequence homology).

Immunogen

Recombinant protein corresponding to mouse Cav3.1.

Application

Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Cav3.1 Ca2+ channel in rat thalamus tissue.

Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in mouse thalamus, cortex, and cerebellum tissue.

Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in rat hippocampus tissue.

Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate.

Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate and in WT mouse brain membrane tissue lysate, but demonstrates a loss of signal in Cav3.1 Ca2+1 knockout mouse brain membrane tissue lysate.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience
This Cav3.1 Ca2+ channel antibody is validated for use in WB & IHC for the detection of the Cav3.1 Ca2+ channel protein.

Quality

Evaluated by Western Blot in mouse cerebellum tissue.

Western Blot Analysis: 1 µg/mL of this antibody from a representative lot detected Cav3.1 Ca2+ channel in 10 µg/mL of mouse cerebellum tissue.

Target description

~230 kDa observed. An uncharacterized band at ~53 kDa may be observed in some tissue lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse cerebellum tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Paula P Perissinotti et al.
Frontiers in neuroscience, 15, 679078-679078 (2021-06-29)
Leptin regulates hypothalamic POMC+ (pro-opiomelanocortin) neurons by inducing TRPC (Transient Receptor Potential Cation) channel-mediate membrane depolarization. The role of TRPC channels in POMC neuron excitability is clearly established; however, it remains unknown whether their activity alone is sufficient to trigger
Marcin Andrzej Lipiec et al.
Development (Cambridge, England), 147(16) (2020-07-18)
Neuronal phenotypes are controlled by terminal selector transcription factors in invertebrates, but only a few examples of such regulators have been provided in vertebrates. We hypothesised that TCF7L2 regulates different stages of postmitotic differentiation in the thalamus, and functions as

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