MABN476

Anti-Paris/ZNF746 Antibody, clone N196/16

clone N196/16, from mouse

• Synonym(s): Zinc finger protein 746, Parkin-interacting substrate, PARIS
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: N196/16, monoclonal
• Application: WB
• Citations: 6

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N196/16, monoclonal
• species reactivity: mouse, human, rat
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001156946
• UniProt accession no.: Q6NUN9
• shipped in: wet ice
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... ZNF746(155061)

Description

General description

Zinc finger protein 746 (ZNF746) is also called Parkin-interacting substrate (PARIS). ZNF746 is a repressor of transcription that binds to promoters and represses the transcription of PGC-1-alpha (PPARGC1A), making it important to the regulation of neuron death. ZNF746 may also contribute to neurodegeneration in Parkinson disease.

~72 kDa observed. Uniprot describes 3 isoforms, two at 69 kDa and one at 49 kDa This protein is ubiquitinated and has been observed at higher molecular weights (Shin, J. H., et al. (2011). Cell. 144(5):689-702.).

Immunogen

Epitope: N-terminus

Recombinant protein corresponding to the N-terminus of human Paris/ZNF746.

Application

Anti-Paris/ZNF746 Antibody, clone N196/16 is a highly specific mouse monoclonal antibody & that targets Zinc Finger Protein & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of RAW264.7 cell lysate. Immunohistochemistry Analysis: A representative lot detected Paris/ZNF746 in rat brain tissue (Prof. J. Trimmer, University of California, Davis.).

Biochem/physiol Actions

This antibody recognizes the N-terminus of ZNF746. This antibody does not cross react with ZNF783 or ZNF398 (Prof. J. Trimmer, University of California, Davis.).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable