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MABN529

Sigma-Aldrich

Anti-Neuronal acetylcholine receptor α-7, clone 6F12.2 Antibody

clone 6F12.2, from mouse

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Synonym(s):
Neuronal acetylcholine receptor subunit alpha-7, acetylcholine receptor alpha-7, Neuronal acetylcholine receptor subunit alpha7, acetylcholine receptor alpha7, nAChRa7, nAChRa-7
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6F12.2, monoclonal

species reactivity

mouse, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHRNA7(1139)

General description

The protein named Neuronal acetylcholine receptor subunit alpha-7, or neuronal nicotinic acetylcholine receptor alpha7 subunit (nAChRa7) is the alpha subunit of a multi-protein, post-synaptic membrane bound ion-channel receptor complex expressed in acetylcholine responsive neurons in the brain and some immune cells. The nAChRa7 receptor belongs to the family of ligand gated ion channels. In the brain, the nAChRa7 receptor is expressed in key brain regions and it is involved in essential cognitive functions such as memory, thinking, comprehension, learning capacity, calculation, orientation, language, and judgment. The a7 subunit has also been identified as a significant mediator of nicotine interactions within the stress axis and human disease. nAChRa7 also has neuroprotective effects via multiple pathways making it a target for treatment of a number of brain disorders including Alzheimer’s disease. Finally, nAChRa7 is key to the cholinergic anti-inflammatory pathway in mediating inflammatory responses by immune cells which can aid in chronic inflammatory diseases like Atherosclerosis.

Immunogen

GST-tagged recombinant protein corresponding to the cytoplasmic domain of Human CHRNA7.

Application

This Anti-Neuronal acetylcholine receptor α-7, clone 6F12.2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Neuronal acetylcholine receptor α-7.
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Neuronal acetylcholine receptor α-7 in 10 µg of Mouse Hypothalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Neuronal acetylcholine receptor α-7 in human cerebellum, cerebral cortex, and thalamic tissue.

Quality

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neuronal acetylcholine receptor α-7 in 10 µg of mouse cerebellum tissue lysate.

Target description

~60 kDa observed. Uncharacterized bands may be observed in some lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Brijesh K Garg et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 65(9), 499-512 (2017-08-02)
Alpha7 nicotinic acetylcholine receptors (α7 nAChRs) are important drug targets in neurological disorders and inflammation, making their detection and localization by validated antibodies highly desirable. However, tests in knockout animals raised questions about specificity of antibodies to mouse α7 nAChRs.

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