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MABN698

Sigma-Aldrich

Anti-FABP2 Antibody, clone 9A9B7B3

ascites fluid, clone 9A9B7B3, from mouse

Synonym(s):

Fatty acid-binding protein, intestinal, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9A9B7B3, monoclonal

species reactivity

human, mouse

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FABP2(2169)

Related Categories

General description

Fatty acid-binding protein 2, (FABP2) is a fatty acid-binding protein produced by the intestinal tissues of the small intestine and participates in the transport and intracellular internalization and metabolism of fatty acids in the gut. Its expression is localized to the small intestine, especially within the cellular epithelial cells of the jejunum, with much lower levels in the large intestine. Its expression is confined to the cytoplasm and induced by growth factors such as EGF. FABP2 is also known as FABPI or I-FABP. Because of its role in fatty acid uptake and lipid homeostasis, mutations or polymorphisms affecting the function of FABP2 in fatty acid metabolism have been a source of recent research. In particular, an amino acid substitution known as A54T where the G to A polymorphism of codon 54 of the FABP2 gene results in the substation of a Thr in the mutated form instead of an Ala in the normal form. This Thr-codon 54 mutation demonstrates an increased affinity for long-chain fatty acids and is strongly associated with factors associated with metabolic syndrome disease, including insulin resistance, enhanced fatty acid oxidation, elevated triglycerides and type 2 diabetes. Furthermore, FABPs and in particular , FABP2, play an increasingly important role in controlling the lipid signaling cascade and FABP2 is being studied as key regulatory factor of lipid signaling under normal and pathological conditions. EMD-Millipore’s anti-FABP2 mouse monoclonal antibody, clone 9A9B7B3, has been tested in western blot, paraffin embedded immunohistochemical, and flow cytometry studies using human tissues and cell lines. The clone also reacts with FABP2 from NIH-3T3-L1 mouse cells lines by immunocytochemistry.

Immunogen

Purified recombinant fragment of human FABP2 expressed in E. Coli.

Application

Detect Fatty Acid Binding Protein using this mouse monoclonal antibody, Anti-FABP2 Antibody, clone 9A9B7B3 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected FABP2 in 3T3-L1 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected FABP2 in human small Intestine tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected FABP2 in LOVO cells.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected FABP2 in human small intestine tissue lysate.

Target description

~15 kDa observed.

Linkage

Replaces: MABS390; MABS401

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Human small intestine tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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