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MABN755

Sigma-Aldrich

Anti-TREM-2 Antibody, clone 78

clone 78, from rat

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Synonym(s):
Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

78, monoclonal

species reactivity

mouse, human, rat

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TREM2(54209)

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This Item
ABN1471MABN1534SAB2501169
Anti-TREM-2 from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN1471

Anti-TREM-2

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

78, monoclonal

clone

polyclonal

clone

10H4.2, monoclonal

clone

polyclonal

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, inhibition assay: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

indirect ELISA: suitable

isotype

IgG1κ

isotype

-

isotype

IgG1κ

isotype

-

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General description

TREM-2, also known as Triggering receptor expressed on myeloid cells 2 or Triggering receptor expressed on monocytes 2, and encoded by the gene Trem2/Trem2a/Trem2b/Trem2c, is a protein that forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. TREM-2 may also have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 is localized to the cell membrane and is a single pass membrane protein. TREM-2 is expressed in immune cells such as microglia and monocytes but the expression varies depending upon the exact tissue and location within the tissue. Defects in TREM-2 may be associated in humans with frontotemporal dementia, Nasu-Hakola disease, and recently an increase risk of Alzheimer′s disease.

Specificity

This antibody does not cross react with mouse TREM-1 or TREM-3 (Prof. William Seaman, University of California, San Francisco.).

Immunogen

Recombinant protein corresponding to mouse TREM-2.

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected TREM-2 in TRACP+ osteoclasts (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).

Flow Cytometry Analysis: A representative lot from an independent laboratory detected TREM-2 in RAW264.7 cells (Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TREM-2 from mouse macrophage lysates (Peng, Q., et al. (2010). Sci Signal. 3(122):ra38.).

Inhibition Analysis: : Pre-treatment of BV2 cells or C57BL/6 Osteoclasts with a representative lot of this antibody significantly reduces cellular activation in response to both untreated and apoptotic Neuro2A cells (Hsieh, C. L., et al. (2009). J Neurochem. (109):4:1144-1156.; Humprey, M. B., et al. (2006). J Bone Miner Res. 21(2):237-245.).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-TREM-2 Antibody, clone 78 is validated for use in western blotting, ICC, flow cytometry, inhibition & IP for the detection of TREM-2.

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.

Target description

~25 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Peng Zhao et al.
Molecular neurodegeneration, 17(1), 44-44 (2022-06-19)
Microglia plays crucial roles in Alzheimer's disease (AD) development. Triggering receptor expressed on myeloid cells 2 (TREM2) in association with DAP12 mediates signaling affecting microglia function. Here we study the negative regulation of TREM2 functions by leukocyte immunoglobulin-like receptor subfamily
Shih-Wei Chen et al.
Methods in molecular biology (Clifton, N.J.), 2683, 53-68 (2023-06-10)
The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for
Yingjun Zhao et al.
Neuron, 97(5), 1023-1031 (2018-03-09)
Mutations in triggering receptor expressed on myeloid cells 2 (TREM2) have been linked to increased Alzheimer's disease (AD) risk. Neurobiological functions of TREM2 and its pathophysiological ligands remain elusive. Here we found that TREM2 directly binds to β-amyloid (Aβ) oligomers

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