Zinc transporter SLC39A7 (UniProt Q92504; also known as Histidine-rich membrane protein Ke4, Really interesting new gene 5 protein, Solute carrier family 39 member 7, ZIP7, Zrt-, Irt-like protein 7) is encoded by the SLC39A7 (also known as HKE4, RING5) gene (Gene ID 7922) in human. Zinc transporters mediate the transport of zinc ions into intracellular compartments as well as the export of excess zinc out of cells. Disruption of zinc homeostasis is responsible for many chronic neurodegenerative diseases and acute neural injuries. Two classes of multi-pass transmembrane Zn transporters are known, including 10 ZnTs and 14 ZIPs, that exhibit opposing functions in mediating zinc homeostasis. ZIP7 serves as a gatekeeper of zinc release from intracellular stores that transports zinc from the endoplasmic reticulum/Golgi apparatus to the cytosol. This transport is stimulated by certain growth calcium, and exogenous zinc. ZIP7 is activated following its phosphorylation at Ser275 and Ser276 by casein kinase 2 (CK2). ZIP7-mediated zinc release from intracellular stores is shown to drive major pathways, such as MAPK, mTOR and PI3K-AKT that are involved in cell survival and proliferation and are activated in cancer cells. Elevated ZIP7 levels are reported in most metastatic melanoma cell lines and in some brain and colon cancer cell lines. Under hyperglycemic conditions, as in diabetes, alterations in ZIP7 phosphorylation have been reported along with opposing changes in the expression of ZIP7 and ZnT7, which induce marked redistribution of cellular free zinc with an increase in cytosolic free zinc and a reduction in sarco(endo)plasmic reticulum. These changes may contribute to cardiac dysfunction commonly observed in diabetes. (Ref.: Nimmanon, T., et al. (2017). Metallomics. 9(5):471-481; Tuncay, E., et al. (2017). Diabetes. 66(5):1346-1358).
Clone 3-3-3 reconizes S256/S257 dual phosphorylated wild-type ZIP7, as well as S256D/S257D, but not S256A/S257A, ZIP7 mutant.
KLH-conjugated phospho-peptide corresponding to the target region sequence with phosphorylated S256 and S257 residues.
Immunocytochemistry Analysis: Clone 3-3-3 hybridoma culture supernatant (1:100 dilution) immunostained 3.7% paraformaldehyde-fixed, 0.4% saponin-permeabilized MCF-7 cells transfected with wild-type and S256D/S257D, but not S256A/S257A, ZIP7 construct (Courtesy of Kathryn Taylor, Ph.D., Cardiff University, United Kingdom).
Western Blotting Analysis: Clone 3-3-3 hybridoma culture supernatant (1:1,000 dilution) detected a ~48 kDa phosphorylated target band in lysate from wild-type ZIP7-transfected MCF-7 cells (Courtesy of Kathryn Taylor, Ph.D., Cardiff University, United Kingdom).
Detect ZIP7 using this mouse monoclonal Anti-phospho-ZIP7 (Ser275/276) Antibody, clone 3-3-3, Cat. No. MABS1262, validated for use in Immunocytochemistry and Western Blotting.
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZIP7 S256/S257 phosphorylation in 20 µg of MCF-7 cell lysate.
~48 kDa observed. 50.12 kDa (human ZIP7) calculated. Uncharacterized bands may be observed in some lysate(s).
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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