MilliporeSigma
All Photos(2)

Documents

MABS1319

Sigma-Aldrich

Anti-Cytochrome P450 26A1 Antibody, clone F27P6A1

clone F27P6A1, from mouse

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
Cytochrome P450 26A1, Cytochrome P450 retinoic acid-inactivating 1, Cytochrome P450RAI, hP450RAI, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

F27P6A1, monoclonal

species reactivity

human

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CYP26A1(1592)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABS497MABS1259SAB4500609
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

F27P6A1, monoclonal

clone

4B7.2, monoclonal

clone

N6-P2H5*G8, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

human

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

isotype

IgG2bκ

isotype

IgG2aκ

isotype

IgG1κ

isotype

-

General description

Cytochrome P450 26A1 (UniProt O43174; also known as Cytochrome P450 retinoic acid-inactivating 1, Cytochrome P450RAI, hP450RAI, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome) is encoded by the CYP26A1 (also known as CYP26, P450RAI1) gene (Gene ID 1592) in human. CYP26A1 is a retinoic acid (RA) metabolizing enzyme that is mainly located in the endoplasmic reticulum membranes and is involved in vitamin A metabolism. It can generate several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. RA is a tight binding ligand for CYP26A1 with low nM binding affinity. Higher expression of CYP26A1 is observed in the adult liver, heart, pituitary gland, placenta, and various regions of the brain. CYP26A1 activity is important for the maintenance of pregnancy, especially during the process of blastocyst implantation. Elevated CYP26A1 expression and RA catabolic activity have been detected in breast epithelial adenocarcinoma cells in culture, in leukemic cells from patients with acute promyelocytic leukemia, and in cells derived from squamous cell carcinoma.

Ref:
Han BC et al., (2010). J. Cell Physiol. 223, 471-479.
Osanai M., and Petkovich M (2005). Mol. Pharmacol. 67, 1808-1817.

Specificity

Target band specificity was confirmed by Western blotting of lysate from cytochrome P450 26A1-overexpressing human embryonic kidney cells (Brown, G.T., et al. (2014). PLoS One. 9(3):e90776).

Immunogen

Ovalbumin-conjugated linear peptide corresponding to a sequence near the C-terminus of human cytochrome P450 26A1 (Kumarakulasingham, M., et al. (2005). Clin. Cancer Res. 11(10):3758-3765).

Application

Anti-Cytochrome P450 26A1, clone F27P6A1, Cat. No. MABS1319, is a highly specific mouse monoclonal antibody that targets Cytochrome P450 26A1 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected cytochrome P450 26A1 in human cerebellum, cerebral cortex, and large intestine tissue sections.

Western Blotting Analysis: 4 µg/mL from a representative lot detected cytochrome P450 26A1 in 10 µg of human liver microsome lysate.

Immunofluorescence Analysis: A representative lot immunostained dentate gyrus MAP2-positive neurons, but not GFAP-positive glia, nor CA1 hippocampal astrocytes and microglia by fluorescent immunohistochemistry staining of formalin-fixed, paraffin-embedded human hippocampus and cerebellum tissue sections (Stoney, P.N., et al. (2015). Brain Struct. Funct. In press).

Immunohistochemistry Analysis: Representative lots detected differential cytochrome P450 26A1 immunoreactivity among formalin-fixed, paraffin-embedded tissue sections from normal colon, colon cancer, and lymph node metastasis (Brown, G.T., et al. (2014). PLoS One. 9(3):e90776; Kumarakulasingham, M., et al. (2005). Clin. Cancer Res. 11(10):3758-3765).

Immunohistochemistry Analysis: A representative lot detected a significantly greater cytochrome P450 26A1 immunoreactivity among ormalin-fixed, paraffin-embedded ovarian cancer tissue sections when compared with normal ovary tissue sections (Downie, D., et al. (2005). Clin. Cancer Res.11(20):7369-7375).

Western Blotting Analysis: A representative lot detected the ~54 kDa cytochrome P450 26A1 target band in human hippocampus tissue homogenate (Stoney, P.N., et al. (2015). Brain Struct. Funct. In press).

Western Blotting Analysis: A representative lot detected the overexpression of cytochrome P450 26A1 exogenously transfected into human embryonic kidney cells (Brown, G.T., et al. (2014). PLoS One. 9(3):e90776).
Research Category
Signaling

Quality

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected cytochrome P450 26A1 in 10 µg of human hippocampus tissue lysate.

Target description

~53/48 kDa observed. 56.20/48.56 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service