MABS15

Anti-IRS-2 Antibody, clone 9.5.2

clone 9.5.2, from mouse

• Synonym(s): insulin receptor substrate 2
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9.5.2, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001074681.1
• UniProt accession no.: P81122
• shipped in: wet ice
• Gene Information: human ... IRS2(8660)

Description

General description

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Specificity

This antibody recognizes IRS-2.

Immunogen

Epitope: Unknown

KLH-conjugated linear peptide corresponding to mouse IRS-2.

Application

Anti-IRS-2 Antibody, clone 9.5.2 detects level of IRS-2 & has been published & validated for use in WB.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Western Blot in Insulin treated CHO IR/IRS-2 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IRS-2 on 10 µg of Insulin treated CHO IR/IRS-2 cell lysate.

Target description

~ 185 kDa

Linkage

Replaces: 06-506

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Insulin treated CHO IR/IRS-2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable