MilliporeSigma
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MABS15

Sigma-Aldrich

Anti-IRS-2 Antibody, clone 9.5.2

clone 9.5.2, from mouse

Synonym(s):
insulin receptor substrate 2
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9.5.2, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... IRS2(8660)

General description

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Specificity

This antibody recognizes IRS-2.

Immunogen

KLH-conjugated linear peptide corresponding to mouse IRS-2.
Epitope: Unknown

Application

Anti-IRS-2 Antibody, clone 9.5.2 detects level of IRS-2 & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Western Blot in Insulin treated CHO IR/IRS-2 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IRS-2 on 10 µg of Insulin treated CHO IR/IRS-2 cell lysate.

Target description

~ 185 kDa

Linkage

Replaces: 06-506

Physical form

Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Insulin treated CHO IR/IRS-2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Alexandra Kuznetsova et al.
JCI insight, 1(3) (2016-05-07)
The capacity of pancreatic β cells to maintain glucose homeostasis during chronic physiologic and immunologic stress is important for cellular and metabolic homeostasis. Insulin receptor substrate 2 (IRS2) is a regulated adapter protein that links the insulin and IGF1 receptors
Patterns of gene and metabolite define the effects of extracellular osmolality on kidney collecting duct.
Hyo-Jung Choi,Yu-Jeong Yoon,Yong-Kook Kwon,Yu-Jung Lee,Sehyun Chae,Daehee Hwang et al.
Journal of Proteome Research null
Gerta Hoxhaj et al.
PloS one, 8(9), e73327-e73327 (2013-09-17)
Insulin receptor substrate 1 (IRS1) and IRS2 are well-characterized adapter proteins that relay signals from receptor tyrosine kinases to downstream components of signalling pathways. In contrast, the function of IRS4 is not well understood. IRS4 overexpression has been associated with
H Ueno et al.
Molecular biology of the cell, 11(2), 735-746 (2000-02-26)
Insulin receptor substrate (IRS) proteins are docking proteins that couple growth factor receptors to various effector molecules, including phosphoinositide-3 kinase, Grb-2, Syp, and Nck. Here we show that IRS-1 associates with the loop domain of Bcl-2 and synergistically up-regulates antiapoptotic
Ori Nov et al.
Endocrinology, 151(9), 4247-4256 (2010-07-28)
Central obesity is frequently associated with adipose tissue inflammation and hepatic insulin resistance. To identify potential individual mediators in this process, we used in vitro systems and assessed if insulin resistance in liver cells could be induced by secreted products

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