MABS1949

Anti-NG2 Antibody, clone 1E6

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• conjugate: unconjugated
• antibody form: purified antibody
• clone: 1E6; monoclonal
• purified by: using protein G
• species reactivity: rat, mouse, mouse
• concentration: (Please refer to lot specific datasheet.)
• technique(s): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: Q8VHY0
• target post-translational modification: unmodified

Description

General description

Oli-Neu cells (murine oligodendroglial precursor cells immortalized by an activated neu tyrosine kinase).

Specificity

Clone 1E6 is a rat monoclonal antibody that detects mouse Chondroitin sulfate proteoglycan 4 (NG2).

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated NG2 in immunoprecipitation applications (Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).

Western Blotting Analysis: A representative lot detected NG2 in Western Blotting applications (Stegmuller, J., et. al. (2002). J Neurocytol. 31(6-7):497-505; Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Trotter, J., et. al. (2005). J Neurol Sci. 233(1-2):37-42; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).

ELISA Analysis: A representative lot detected NG2 in ELISA applications (Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).

Immunofluorescence Analysis: A representative lot detected NG2 in Immunoprecipitation applications (Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Karram, K., et. al. (2005). J Anat. 207(6):735-44; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).

Immunohistochemistry Analysis: A representative lot detected NG2 in Immunohistochemistry applications (Karram, K., et. al. (2005). J Anat. 207(6):735-44; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61).

Quality

Evaluated by Western Blotting in mouse brain tissue lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected NG2 in mouse brain tissue lysates.

Physical properties

~330 kDa observed; 252.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.