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MABS1984

Sigma-Aldrich

Anti-INTS1 Antibody, clone 4.47

clone 4.47, from mouse

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Synonym(s):
Integrator complex subunit 1, Int1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4.47, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... INTS1(26173)

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HPA021658SAB4301061HPA053009
Anti-INTS1 Antibody, clone 4.47 clone 4.47, from mouse

MABS1984

Anti-INTS1 Antibody, clone 4.47

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

human

species reactivity

mouse, human

species reactivity

human

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

4.47, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: 1:500-1:1000, western blot: 0.04-0.4 μg/mL

technique(s)

immunohistochemistry: 1:25- 1:100

technique(s)

immunofluorescence: 0.25-2 μg/mL

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General description

Integrator complex subunit 1 (UniProt Q8N201; also known as Int1) is encoded by the INTS1 (also known as KIAA1440, UNQ1821/PRO3434) gene (Gene ID 26173) in human. INTS1 is a large protein that is a component of the integrator complex that is involved in small nuclear RNAs U1 and U2 transcription and their 3′-box-dependent processing. During transcriptional regulation and in RNA processing, it directly binds to the C-terminal domain of the RNA polymerase II largest subunit. It has been reported that INTS1 is also involved in different cell support processes, such as the formation of a scaffold for the assembly of integrator complex. Developing INTS1 (-/-) mouse embryos show growth arrest at the early blastocyst stage and exhibit activated caspase-3 and caspase-7 that induce apoptosis within the inner cell mass of blastocyst.

Ref.:
Baillat, D et al. (2005). Cell 123, 265-276.
Hata T., and Nakayama, M (2007), Biochim. Biophys. Acta 1773, 1039-1051.

Specificity

Clone 4.47 targets an epitope in the central region of human INTS1 protein.

Immunogen

GST-tagged recombinant human INTS1 internal fragment.

Application

Anti-INTS1, clone 4.47 Antibody, Cat. No. MABS1984, is a highly specific mouse monoclonal antibody, that targets INTS1 and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected in 50 µg of HeLa cell lysate.

Target description

~240 kDa observed. 244.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Felipe Beckedorff et al.
Cell reports, 32(3), 107917-107917 (2020-07-23)
Transcription by RNA polymerase II (RNAPII) is pervasive in the human genome. However, the mechanisms controlling transcription at promoters and enhancers remain enigmatic. Here, we demonstrate that Integrator subunit 11 (INTS11), the catalytic subunit of the Integrator complex, regulates transcription
Jasmine Barra et al.
Science advances, 6(27), eaaz9072-eaaz9072 (2020-09-15)
RNA 3' end processing provides a source of transcriptome diversification which affects various (patho)-physiological processes. A prime example is the transcript isoform switch that leads to the read-through expression of the long non-coding RNA NEAT1_2, at the expense of the
Stephin J Vervoort et al.
Cell, 184(12), 3143-3162 (2021-05-19)
Gene expression by RNA polymerase II (RNAPII) is tightly controlled by cyclin-dependent kinases (CDKs) at discrete checkpoints during the transcription cycle. The pausing checkpoint following transcription initiation is primarily controlled by CDK9. We discovered that CDK9-mediated, RNAPII-driven transcription is functionally

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