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MABS2005

Sigma-Aldrich

Anti-2A peptide Antibody, clone 3H4

clone 3H4, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

3H4, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

shipped in

ambient

target post-translational modification

unmodified

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antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

3H4, monoclonal

clone

5E6, monoclonal

clone

3H4, recombinant monoclonal

clone

recombinant monoclonal

species reactivity

mouse

species reactivity

all

species reactivity

rat, monkey, human

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 100 μg

packaging

antibody small pack of 25 μL

packaging

antibody small pack of 25 μL

technique(s)

immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

General description

2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 20 amino acids (depending on the virus of origin) containing the consensus motif Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro. 2A peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multicistronic construct. 2A peptides are used by several families of viruses, the best known foot-and-mouth disease virus of the Picornaviridae family, for producing multiple polypeptides. Anti-2A peptide, clone 3H4 recognizes 2A sequence derived from foot and mouth piconavirus (VKQTLNFDLLKLAGDVESNPG*P) with cleavage site between G and P. (Ref.: Trichas, G, et al. (2008). BMC Biol. 6: 40).

Specificity

Clone 3H4 detects 2A peptide in murine cells.

Immunogen

KLH-conjuated linear peptide corresponding to sequence CGDVEENPG (free C-term) derived from Thosea asigna virus.

Application

Anti-2A peptide, clone 3H4, Cat. No. MABS2005, is a mouse monoclonal antibody that detects 2A peptide and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Research Category
Secondary & Control Antibodies
Western Blotting Analysis: A representative lot detected 2A peptide in HEK293 SpCas9-P2A-Puro and NIH3T3 SpCas9-T2A-Puro cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot detected 2A peptide in HeLa cells expressing SpCas9-P2A or plain HeLa cells, HEK293 cells expressing SpCas9-T2A or plain HEK293 cells (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunofluorescence Analysis: A representative lot detected 2A peptide in HeLa cells transiently transfected with SaCas9-T2A-GFP (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Quality

Evaluated by Western Blotting in NIH3T3 expressing SpCas9-T2A-Puro cell lysate.

Western Blotting Analysis: 0.5 ug/mL of this antibody detected 2A peptide in 10 µg of NIH3T3 expressing SpCas9-T2A-Puro cell lysate.

Target description

~150 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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