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MABS2028

Sigma-Aldrich

Anti-Vitamin D receptor Antibody, clone 9A7

clone 9A7, from rat

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Synonym(s):
VDR, 1, 25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9A7, monoclonal

species reactivity

chicken, rat, porcine, human

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

chicken ... Vdr(395988)
human ... VDR(7421)

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MABN1601WH0007421M2GR37
clone

9A7, monoclonal

clone

6F3.1, monoclonal

clone

2F4, monoclonal

clone

9A7γE10.4, monoclonal

biological source

rat

biological source

mouse

biological source

mouse

biological source

rat

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

Gene Information

chicken ... Vdr(395988)
human ... VDR(7421)

Gene Information

human ... VDR (7421)

Gene Information

human ... VDR(7421)

Gene Information

human ... VDR(7421)

UniProt accession no.

O42392

UniProt accession no.

P11473

UniProt accession no.

P11473

UniProt accession no.

-

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General description

Vitamin D3 receptor (UniProt: O42392; also known as VDR, 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1) is encoded by the VDR (also known as NR1I1) gene (Gene ID: 395988) in chicken. VDR is a nuclear receptor for calcitriol, the active form of vitamin D3 that mediates the action of vitamin D on cells. VDR is expressed mainly in kidney and intestine and plays a central role in calcium homeostasis. In the absence of bound vitamin D3 it is present as a homodimer and it enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. It is recruited to promoters via its interaction with BAZ1B/WSTF, which mediates the interaction with acetylated histones that is considered to be an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. VDR is composed of three domains: a modulating N-terminal domain, a DNA-binding domain, and a C-terminal ligand-binding domain. Two isoforms of VDR have been described that are produced by alternative initiation.

Specificity

Clone 9A7 detects Vitamin D3 receptor in multiple avian and mammalian species.

Immunogen

Partially purified chicken intestinal vitamin D receptor.

Application

Anti-Vitamin D receptor, clone 9A7, Cat. No. MABS2028, is a rat monoclonal antibody that detects Vitamin D3 receptor and has been tested for use in , Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Vitamin D receptor in Immunoprecipitation applications (Pike, J.W., et. al. (1982). Proc Natl Acad Sci USA. 79(24); 7719-23).

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Vitamin D receptor in human skin and human breast cancer tissue sections.
Research Category
Signaling

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Vitamin D receptor in MCF-7 cell lysate.

Target description

~51 kDa observed; 51.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 04-1526

Physical form

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Zixian Huang et al.
Cell death & disease, 10(12), 936-936 (2019-12-11)
Chemoresistance is a major cause of cancer progression and the mortality of cancer patients. Developing a safe strategy for enhancing chemosensitivity is a challenge for biomedical science. Recent studies have suggested that vitamin D supplementation may decrease the risk of

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