MilliporeSigma
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MABS412

Sigma-Aldrich

Anti-EphA2 Antibody, clone SHM16

clone SHM16, from mouse

Synonym(s):
Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

SHM16, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

wet ice

Gene Information

human ... EPHA2(1969)

General description

EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptor A2 (EphA2), also known as Eck, is a Receptor tyrosine kinase activated by the ligand ephrin-A1/EFNA1 that regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Additioanally, EphA2 regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. EphA2 may also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. Recent studies have shown that during development, EphA2 may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Additioanally, it interacts with ligand ephrin-A5/EFNA5 and may regulate lens fiber cells shape, lens transparency development and maintenance. EphA2 has been suggested to play in importnat role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis druing its interaction with ephrin-A2/EFNA2 . Mutations in EphA2 have been associated with Cataract 6, multiple types (CTRCT6): an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Detect EphA2 using this mouse monoclonal antibody, Anti-EphA2 Antibody & clone SHM16 validated for use in Flow Cytometry.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

Flow Cytometry Analysis: 1 μg of this antibody detected EphA2 in one million A375 cells.

Target description

108 kDa calculated

Physical form

Affinity
Format: Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.

Storage and Stability

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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