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MABS497

Sigma-Aldrich

Anti-Cytochrome P450 26B1 Antibody, clone 4B7.2

clone 4B7.2, from mouse

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Synonym(s):
Cytochrome P450 26B1, Cytochrome P450 26A2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450, subfamily XXVIB, polypeptide 1, Cytochrome P450RAI-2, Retinoic acid-metabolizing cytochrome
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4B7.2, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CYP26B1(56603)

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MABS1319MABS1259AB10320
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

serum

clone

4B7.2, monoclonal

clone

F27P6A1, monoclonal

clone

N6-P2H5*G8, monoclonal

clone

polyclonal

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

isotype

IgG2aκ

isotype

IgG2bκ

isotype

IgG1κ

isotype

-

General description

Cytochrome P450 26B1 (UniProt Q9NR63; also known as Cytochrome P450 26A2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450, subfamily XXVIB, polypeptide 1, Cytochrome P450RAI-2, Retinoic acid-metabolizing cytochrome) is encoded by the CYP26B1 (also known as CYP26A2, P450RAI2, RHFCA) gene (Gene ID 56603) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum. In addition to processing endogenous substrates, CYPs also metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consists of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. The CYP26 subfamily of enzymes (CYP26A1, CYP26B1/CYP26A2, CYP26C1) are retinoic acid hydroxylases responsible for the inactivation of all-trans-retinoic acid to hydroxylated forms, such as 4-oxo-, 4-OH-, and 18-OH-all-trans-retinoic acid. CYP26B1 is overexpressed in colorectal cancer (CRC) cells, high CYP26B1 expression level in CRC patients is associated with poor prognosis. CYP26B1 mutations are found in radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA), a disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects.

Specificity

Expected to react with all three spliced isoforms of human CYP26B1 reported by UniProt (Q9NR63).

Immunogen

Epitope: Internal (C-terminal half).
GST-tagged recombinant internal fragment from the C-terminal half of human Cytochrome P450 26B1.

Application

Anti-Cytochrome P450 26B1 Antibody, clone 4B7.2 is an antibody against Cytochrome P450 26B1 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytochrome P450 26B1 in human brain, mouse brain, and rat cerebellum tissue.
Research Category
Signaling
Research Sub Category
Toxicology & Drug Resistance

Quality

Evaluated by Western Blotting in mouse brain tissue microsomal lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Cytochrome P450 26B1 in 10 µg of mouse brain tissue microsomal lysate.

Target description

~57 kDa observed. 57.51 kDa (isoform 1), 49.01 kDa (isoform 2), and 55.76 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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