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MABT1346

Sigma-Aldrich

Anti-P-Cadherin Antibody, clone CSTEM29

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Synonym(s):
Cadherin-3;Placental Cadherin

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

CSTEM29, monoclonal

mol wt

calculated mol wt 91.42 kDa
observed mol wt ~80 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μg

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG2aκ

epitope sequence

Extracellular domain

Protein ID accession no.

UniProt accession no.

shipped in

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CHD3(1001)

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

hamster, human

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

UniProt accession no.

 P22223

UniProt accession no.

P55285

UniProt accession no.

Q14126

UniProt accession no.

P19022

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General description

Cadherin-3 (UniProt: P22223; also known as Placental cadherin, P-cadherin) is encoded by the CHD3 (also known as CDHP) gene (Gene ID: 1001) in human. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. P-cadherin is a class I cadherin that is expressed in myoepithelial cells and is also expressed during late pregnancy and lactation, when luminal epithelial cells secrete high levels of a soluble fragment of P-cadherin in human milk. It is a single pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24) and a propeptide (aa 25-107 that are subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 108-654), a transmembrane domain (aa 655-677), and a cytoplasmic domain (aa 678-829). It has 5 cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections and thereby imparting a strong curvature to the full-length ectodomain. P-cadherin dysfunction is strongly associated with tumorigenesis and confers the malignant phenotype in cancer cells. Its overexpression is strongly associated with poor prognosis in several solid tumors. Its high expression is reported to be predictive of shorter overall survival in glioblastoma patients. It is also detected in plasma membrane during progression of pancreatic intraepithelial ductal adenocarcinoma. (Ref.: Martins, EP., et al. (2021). Mol. Oncol. doi: 10.1002/1878-0261; Yu, W., et al. (2019). Front. Oncol. 9; 989; Siret, C., et al. (2018). Br. J. Cancer. 118; 546-557).

Specificity

Clone CSTEM3 is a mouse monoclonal antibody that detects P-Cadherin (Cadherin 3). It targets an epitope within the extracellular domain.

Immunogen

His-tagged recombinant protein fragment corresponding to the extracellular domain of human P-Cadherin (Cadherin 3).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human P-Cadherin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human P-Cadherin.

Tested Applications

ELISA Analysis: A representative lot detected P-Cadherin in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Flow Cytometry Analysis: A representative lot detected P-Cadherin in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected P-Cadherin in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-P-Cadherin, clone CSTEM29, Cat. No. MABT1346, is a mouse monoclonal antibody that detects P-Cadherin and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


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