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MABT1527

Sigma-Aldrich

Anti-CLM-9 (CD300g), clone ZAQ2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
Pricing and availability is not currently available.

antibody form

purified antibody

antibody product type

primary antibodies

clone

ZAQ2
monoclonal

mol wt

(~ 32 kDa observed; 36.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

purified by

using protein G

species reactivity

mouse, rat

isotype

IgG2aκ

Protein ID accession no.

UniProt accession no.

Specificity

Clone ZAQ2 is a rat monoclonal antibody that detects murine CMRF35-like molecule 9 (CLM-9).

Application

Anti-CLM-9 (CD300g), clone ZAQ2, Cat. No. MABT1527, is a rat monoclonal antibody that detects CMRF35-like molecule 9 (CD300g) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
ELISA Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Western Blotting Analysis: A representative lot detected CLM-9 (CD300g) in Western Blotting applications (Umemoto, E., et. al. (2006). J. Exp. Med. 203(6):1603-14).

ELISA Analysis: A representative lot detected CLM-9 (CD300g) in ELISA applications (Umemoto, E., et. al. (2006). J. Exp. Med. 203(6):1603-14).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLM-9 (CD300g) in Immunohistochemistry applications (Umemoto, E., et. al. (2006). J. Exp. Med. 203(6):1603-14; Umemoto, E., et. al. (2013). PLos One. 8(12):e83681).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CLM-9 (CD300g) in Immunoprecipitation applications (Umemoto, E., et. al. (2006). J. Exp. Med. 203(6):1603-14).

Features and Benefits

Extracellular domain of mouse CLM-9 (Nepmucin) protein fused with the Fc portion of human IgG.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Other Notes

CMRF35-like molecule 9 (UniProt: Q1ERP8; also known as CLM-9, CD300 antigen-like family member G, Nepmucin, CD300g, CD300LG) is encoded by the Cd300lg (also known as Clm9, D11Ertd736e) gene (Gene ID: 52685) in murine species. CLM-9 is a single-pass type I membrane protein that serves as a receptor that mediates L-selectin-dependent lymphocyte rollings. It is highly expressed in the heart tissue and its expression has also been reported in monocyte cell lines, certain types of endothelial and myeloid lineage cells. Isoform 5 is expressed preferentially in heart and isoform 1 is expressed predominantly in kidney and liver. Its expression is shown to decline rapidly in lymph nodes after receiving acute inflammatory signals and this loss is shown to be mediated partially by TNF-alpha. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 19-204), a transmembrane domain (aa 205-224), and a cytoplasmic domain (aa 226-331). Post-translationally, it undergoes O-glycosylation on multiple sites with sialylated oligosaccharides. (Ref.: Umemoto, E., et al. (2013). PLoS ONE 8(12); e83681).
Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CLM-9 (CD300g) in mouse spleen tissue lysate.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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