MABT201

Anti-Integrin alpha 5 (ITGA5/CD49E) Antibody

mouse monoclonal, SNAKA51

• Synonym(s): Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: SNAKA51, monoclonal
• Species reactivity: human
• Application: ICC, WB, activity assay
• Citations: 10

Properties

• Product Name: Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51, clone SNAKA51, from mouse, purified by using protein G
• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SNAKA51, monoclonal
• purified by: using protein G
• species reactivity: human
• technique(s): activity assay: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2α
• NCBI accession no.: NP_001138472
• UniProt accession no.: P08648
• shipped in: wet ice
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... ITGAV(3685)

Description

General description

116 kDa calculated

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The α5/β1 integrin, also termed VLA5 or CD49e, is identical to the fibronectin receptor and mediates the adhesion of cells to fibronectin.

Immunogen

Whole cells corresponding to human Integrin alpha5.

Application

Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.).
Immunocytochemistry Analysis: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF) (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.; Valdembri, D., et al. (2009). PLoS Biol. 7(1):e25.).

Detect Integrin alpha5 (Preservative Free) using this Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 validated for use in Western Blotting, Activity Assay, ICC.

Research Category
Cell Structure

Physical form

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.

Analysis Note

Control
HeLa cells

Evaluated by Flow Cytometry in HeLa cell treated with fibronectin.
Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha5 in 0.5X10^6 HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable