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MABT372

Sigma-Aldrich

Anti-Mastl Antibody, clone 4F9

clone 4F9, from mouse

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Synonym(s):
Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F9, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MASTL(84930)

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HPA027175WH0023139M1HPA000544
vibrant-m

MABT372

Anti-Mastl Antibody, clone 4F9

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

Gene Information

human ... MASTL(84930)

Gene Information

human ... MASTL(84930)

Gene Information

human ... MAST2(23139)

Gene Information

human ... MAST4(375449)

UniProt accession no.

Q96GX5

UniProt accession no.

Q96GX5

UniProt accession no.

Q6P0Q8

UniProt accession no.

O15021

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General description

Mastl is also called Serine/threonine-protein kinase greatwall (GW) and Microtubule-associated serine/threonine-protein kinase-like (MAST-L). Mastl is a serine/threonine kinase that regulates mitosis entry and maintenance during M phase through the inactivation of protein phosphatase 2A (PP2A). Mastl is also thought to be involved in megakaryocyte differentiation and the diseaseThrombocytopenia 2 (THC2).

Immunogen

Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of human Mastl.

Application

Detect Mastl using this mouse monoclonal antibody, Anti-Mastl Antibody, clone 4F9 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected Mastl in human prostate cancer and human testis tissue sections.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in human oral squamos cell carcinoma lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Mastl in 10 µg of human oral squamos cell carcinoma lysate.

Target description

~97 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Srijayaprakash Babu Uppada et al.
Molecular cancer, 17(1), 111-111 (2018-08-03)
Chemotherapeutic agents that modulate cell cycle checkpoints and/or tumor-specific pathways have shown immense promise in preclinical and clinical studies aimed at anti-cancer therapy. MASTL (Greatwall in Xenopus and Drosophila), a serine/threonine kinase controls the final G2/M checkpoint and prevents premature
Odjo G Gouttia et al.
Frontiers in cell and developmental biology, 10, 904719-904719 (2022-10-18)
Platinum-based chemotherapy is the standard first-line treatment for oral squamous cell carcinoma (OSCC) that is inoperable, recurrent, or metastatic. Platinum sensitivity is a major determinant of patient survival in advanced OSCC. Here, we investigated the involvement of MASTL, a cell
Dario Hermida et al.
Molecular & cellular proteomics : MCP, 19(2), 326-343 (2019-12-20)
The human MASTL (Microtubule-associated serine/threonine kinase-like) gene encodes an essential protein in the cell cycle. MASTL is a key factor preventing early dephosphorylation of M-phase targets of Cdk1/CycB. Little is known about the mechanism of MASTL activation and regulation. MASTL
Yanqiu Li et al.
eLife, 12 (2023-09-06)
Checkpoint activation after DNA damage causes a transient cell cycle arrest by suppressing cyclin-dependent kinases (CDKs). However, it remains largely elusive how cell cycle recovery is initiated after DNA damage. In this study, we discovered the upregulated protein level of
Dapeng Ren et al.
The Journal of biological chemistry, 292(24), 10026-10034 (2017-04-28)
Greatwall (Gwl) kinase plays an essential role in the regulation of mitotic entry and progression. Mitotic activation of Gwl requires both cyclin-dependent kinase 1 (CDK1)-dependent phosphorylation and its autophosphorylation at an evolutionarily conserved serine residue near the carboxyl terminus (Ser-883

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