MABT538

Anti-Lamin A/C Antibody, clone 2E8.2

clone 2E8.2, from mouse

• Synonym(s): Prelamin-A/C, Lamin-A/C, Renal carcinoma antigen NY-REN-32
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E8.2, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_733821
• UniProt accession no.: P02545
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... LMNA(4000)

Description

General description

Lamin-A is a key component of the nuclear lamina, a protein network underlying the inner membrane that determines nuclear shape and size. The same gene (Lmna, Dhe, or Lamn1 in murine species; Gene ID 16905) that encodes lamin-A also codes for the alternatively spliced prelamin-C isoforms (UniProt P48678-2 and P48678-3). Murine lamin-A (UniProt P48678-1) is initially produced as a 665-amino acid prelamin A, which is further processed posttranslationally by four enzymatic steps to yield the mature Lamin-A. Farnesylation of Cys662, endoproteolytic cleavage of the last three amino acids (a.a. 663-665), carboxyl methylation of Cys662, and the final endoproteolytic removal of the remaining propeptide sequence (a.a. 648-662), including the farnesylated Cys662, catalyzed by the ER membrane zinc metalloproteinase ZMPSTE24.

~70/65 kDa observed. 74.14/73.81/72.22 kDa (perpro-/pre-/mature Lamin A) and 65.14 kDa (Lamin C) calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

GST-tagged recombinant fragment within the C-terminal half of human Lamin A.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained nuclear membrane in human cerebellum, kidney, and pancreas tissue sections.

Research Category
Cell Structure

This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Cat. No. MABT538 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of lamin A/C.

Biochem/physiol Actions

Clone 2E8.2 immunostained nuclear membrane in human tissue sections.

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected lamin A/C in 10 µg of A431 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: MAB3211

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1