MilliporeSigma
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MABT837

Sigma-Aldrich

Anti-LBPA Antibody, clone 6C4

clone 6C4, from mouse

Synonym(s):
LBPA
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6C4, monoclonal

species reactivity (predicted by homology)

all

technique(s)

ELISA: suitable
dot blot: suitable
electron microscopy: suitable
immunocytochemistry: suitable

isotype

IgG1κ

shipped in

wet ice

Gene Information

bacterial ... Lbpa(61281717)

General description

Lyso bis-phosphatidic acid (LBPA, Lysobisphosphatidic acid, L(bis)PA) is a lipid that is exclusively located in late endosomes and is used as a late endosome marker. One of the characteristic features of late endosomes is a complex system of internal membranes within the lumen that contains large amounts of the unique, poorly degradable LBPA, and thus forms a specialized membrane domain within endosomes. Late endosomes function not only as a major protein-sorting compartment, but also an obligatory station for LDL and other endocytosed ligands destined for degradation. Research shows that the LBPA-rich membranes within late endosomes regulate cholesterol transport, presumably by acting as a collection and distribution device. The genetic disease Niemann–Pick type C (NPC) is characterized by both lysosomal and endosomal storage disorders. In skin fibroblasts from NPC patients, cholesterol accumulation is seen within vesicles containing late endosome markers LBPA and Rab7.

Specificity

Target molecule is not species-specific.
Clone 6C4 specifically detects LBPA, but not PC, PE, SM, PS, CL, PI, Sul, Chol, Cer, or CE purified from baby hamster kidney (BHK) cell lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).

Immunogen

Endosomal membranes from baby hamster kidney fibroblasts (BHK) corresponding to Hamster LBPA.

Application

Research Category
Cell Structure
Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected LBPA in A431, HUVEC and NIH/3T3 cells.
Immunocytochemistry Analysis: A representative lot detected late endosome LBPA immunoreactivity by fluorescent immunocytochemistry using MLN64-overexpressing MCF7 cells (Alpy, F., et al. (2001). J Biol Chem. 276(6):4261-4269).
Immunocytochemistry Analysis: A representative lot detected late endosome LBPA immunoreactivity in skin fibroblasts from healthy donors, as well as Niemann–Pick type C (NPC) and Tay-Sachs disease patients by fluorescent immunocytochemistry (Kobayahsi, T., et al. (1999). Nat Cell Biol. 1(2):113-118).
Immunocytochemistry Analysis: A representative lot detected late endosome LBPA immunoreactivity in baby hamster kidney (BHK) cells by fluorescent immunocytochemistry (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
Electron Microscopy Analysis: A representative lot detected similar cellular LBPA distribution in the skin fibroblasts from autosomal recessive Niemann–Pick type C disease (NPC) patients as seen in baby hamster kidney (BHK) cells (Kobayahsi, T., et al. (1999). Nat Cell Biol. 1(2):113-118).
Electron Microscopy Analysis: A representative lot detected LBPA immunoreactivity specifically associated with the late endosomes in baby hamster kidney (BHK) cells, but not the lgp120-positive limiting membranes of the same endosomes (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
ELISA Analysis: A representative lot specifically detected wells coated with total baby hamster kidney (BHK) cell lipid extract or purified LBPA, but not other lipids (PC, PE, SM, PS, CL, or PI) purified BHK lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197)
Dot Blot Analysis: A representative lot specifically detected purified LBPA spotted on a HPTLC plate, but not other lipids (PC, PE, SM, PS, CL, PI, Sul, Chol, Cer, CE) purified from baby hamster kidney (BHK) cell lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
This Anti-LBPA Antibody, clone 6C4 is validated for use in Immunocytochemistry, Electron Microscopy, ELISA and Dot Blot for the detection of LBPA.
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody stained late endosomes in HeLa cells.

Physical form

Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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