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MADCYMAG-72K

Millipore

MILLIPLEX® Mouse Adipocyte Magnetic Panel - Endocrine Multiplex Assay

The analytes available for this multiplex kit are: Adiponectin, IL-6, Leptin, MCP-1, PAI-1 (Total), Resistin, TNF-α.

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eCl@ss:
32161000

Quality Level

200

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 85-98%
standard curve range: 12.2-50,000 pg/mL

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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MADKMAG-71KHADCYMAG-61KHADK1MAG-61K
MILLIPLEX® Mouse Adipocyte Magnetic Panel - Endocrine Multiplex Assay The analytes available for this multiplex kit are: Adiponectin, IL-6, Leptin, MCP-1, PAI-1 (Total), Resistin, TNF-α.

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MILLIPLEX® Mouse Adipocyte Magnetic Panel - Endocrine Multiplex Assay

MILLIPLEX® Mouse Adipokine Magnetic Bead Panel - Endocrine Multiplex Assay The analytes available for this multiplex kit are: IL-6, Insulin, Leptin, MCP-1, PAI-1 (Total), Resistin, TNF-α.

Millipore

MADKMAG-71K

MILLIPLEX® Mouse Adipokine Magnetic Bead Panel - Endocrine Multiplex Assay

MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.

Millipore

HADCYMAG-61K

MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay

MILLIPLEX® Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay The Human Adipokine Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of five biomarkers in human serum or plasma samples.

Millipore

HADK1MAG-61K

MILLIPLEX® Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 85-98%, standard curve range: 12.2-50,000 pg/mL

assay range

accuracy: 77-92%, standard curve range: 12.2-50,000 pg/mL

assay range

accuracy: 100%
(IL-6), accuracy: 102%
(TNFα), accuracy: 103%
(MCP-1), accuracy: 83%
(PAI-1 (total)), accuracy: 99%
(HGF), sensitivity: 0.2 pg/mL
(MinDC+2SD; IL-8), sensitivity: 0.2 pg/mL
(MinDC+2SD; NGF), sensitivity: 0.2 pg/mL
(MinDC+2SD; TNF?), sensitivity: 0.6 pg/mL
(MinDC+2SD; IL-6), sensitivity: 10 pg/mL
(MinDC+2SD; Leptin), sensitivity: 15 pg/mL
(MinDC+2SD; Adiponectin), sensitivity: 2.0 pg/mL
(MinDC+2SD; IL-1?), sensitivity: 2.1 pg/mL
(MinDC+2SD; MCP-1), sensitivity: 3.0 pg/mL
(MinDC+2SD; HGF), sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin), sensitivity: 4.6 pg/mL
(MinDC+2SD; PAI-1 (total)), standard curve range: 0.29-4,000 pg/mL
(IL-8), standard curve range: 0.32-5,000 pg/mL
(NGF), standard curve range: 0.38-6,000 pg/mL
(TNFα), standard curve range: 0.51-8,000 pg/mL
(IL-1β), standard curve range: 0.96-150,000 pg/mL
(IL-6), standard curve range: 0.96-150,000 pg/mL
(MCP-1), standard curve range: 26-400,000 pg/mL
(Adipnectin), standard curve range: 6.4-100,000 pg/mL
(HGF), standard curve range: 6.4-100,000 pg/mL
(Resistin), standard curve range: 9.6-150,000 pg/mL
(Leptin), standard curve range: 9.6-150,000 pg/mL
(PAI-1), inter-assay cv: <15%
intra-assay cv: <10%
(Adiponectin), inter-assay cv: <15%
intra-assay cv: <10%
(IL-8), inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1), inter-assay cv: <15%
intra-assay cv: <10%
(Resistin), inter-assay cv: <20%
intra-assay cv: <10%
(HGF), inter-assay cv: <20%
intra-assay cv: <10%
(IL-6), inter-assay cv: <20%
intra-assay cv: <10%
(NGF), inter-assay cv: <20%
intra-assay cv: <10%
(PAI-1 (total)), inter-assay cv: <20%
intra-assay cv: <10%
(TNFα), inter-assay cv: <20%
intra-assay cv: <15%
(IL-1β), inter-assay cv: <25%
intra-assay cv: <20%
(Leptin)

assay range

accuracy: 87%
(Resistin), accuracy: 89%
(Adiponectin), sensitivity: 10 pg/mL
(MinDC+2SD; Adipsin), sensitivity: 21 pg/mL
(MinDC+2SD; Adiponectin), sensitivity: 3.5 pg/mL
(MinDC+2SD; Lipocalin-2/NGAL), sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin), sensitivity: 5.8 pg/mL
(MinDC+2SD; PAI-1), standard curve range: 13-200,000 pg/mL
(Adipsin), standard curve range: 26-400,000 pg/mL
(Adiponectin), standard curve range: 3.2-50,000 pg/mL
(Lipocalin-2/NGAL), standard curve range: 6.4-100,000 pg/mL
(Resistin), standard curve range: 9.6-150,000 pg/mL
(PAI-1 (total)), inter-assay cv: <10%
intra-assay cv: <10%
(Adipsin), inter-assay cv: <10%
intra-assay cv: <15%
(Adiponectin), inter-assay cv: <10%
intra-assay cv: <15%
(Lipocalin-2/NGAL), inter-assay cv: <10%
intra-assay cv: <20%
(PAI-1 (total)), inter-assay cv: <10%
intra-assay cv: <20%
(Resistin)

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of leptin secretion from adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these adipokines, adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Its anti-inflammatory effects on the cellular lining of blood vessel walls may explain the association of high adiponectin levels with a reduced risk of heart attack.

MILLIPLEX® Mouse Adipocyte Panel is a 7-plex kits used for the simultaneous quantification of any or all of the following analytes in mouse tissue culture supernatant samples: Adiponectin, IL-6, Leptin, MCP-1, PAI-1 Total, Resistin and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

  • Analytes: Adiponectin, IL-6, Leptin, MCP-1, PAI-1 (Total), Resistin, TNFα
  • Recommended Sample Type: Mouse tissue culture supernatants
  • Recommended Sample Dilution: 10 μL per well of tissue culture supernatant; dilution with an appropriate control medium may be required.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C).
  • Research Category: Metabolism
  • Research Subcategory: Endocrine, Obesity, Metabolic Disorders

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Sensitivity: Please see kit protocol for sensitive’s of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

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MMYOMAG-74K-13

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Gabriela Martínez-Chacón et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(5), e21536-e21536 (2021-04-30)
Obesity is a risk factor for postmenopausal breast cancer. Obesity-related inflammation upregulates aromatase expression, the rate-limiting enzyme for estrogen synthesis, in breast adipose tissue (BAT), increasing estrogen production and cancer risk. The regulation of aromatase gene (CYP19A1) in BAT is
Nasser M Rizk et al.
Journal of inflammation research, 14, 1743-1757 (2021-05-14)
Obesity is a chronic disorder characterized by a low-grade inflammatory state and immune cell irregularities. The study aimed to follow up on the changes in the peripheral CD4+ T lymphocytes and the pro-inflammatory cytokines; IL-6, TNF-alpha, MCP-1, and IL-10 at
Clément L'Huillier et al.
Nutrients, 11(6) (2019-06-19)
During activity-based anorexia (ABA) in mice, enhanced paracellular permeability and reduced protein synthesis have been shown in the colon while the gut-brain axis has received increasing attention in the regulation of intestinal and mood disorders that frequently occur during anorexia
Karima Drareni et al.
Cell reports, 32(11), 108141-108141 (2020-09-17)
Glucose homeostasis is maintained through organ crosstalk that regulates secretion of insulin to keep blood glucose levels within a physiological range. In type 2 diabetes, this coordinated response is altered, leading to a deregulation of beta cell function and inadequate

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