MilliporeSigma
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MAP2MAG-76K

Millipore

MILLIPLEX® Mouse Acute Phase Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

The analytes available for this multiplex kit are: Adipsin, Alpha-1 Acid Glycoprotein (AGP), Alpha-2 Macroglobulin (A2M), C-Reactive Protein, Haptoglobin, SAP.

eCl@ss:
32161000

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 99.2-128.2%
sensitivity: 0.001-7.495 ng/mL
standard curve range: 0.001-0.5 ng/mL
(AGP)

standard curve range: 0.005-4.0 ng/mL
(Adipsin)

standard curve range: 0.014-10 ng/mL
(CRP)

standard curve range: 0.549-400 ng/mL
(Haptoglobin)

standard curve range: 13.717-10,000 ng/mL
(SAP)

standard curve range: 6.858-5000 ng/mL
(A2M)

inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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MKI1MAG-94KMADKMAG-71KMCVD2MAG-77K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 99.2-128.2%, sensitivity: 0.001-7.495 ng/mL, standard curve range: 0.001-0.5 ng/mL
(AGP), standard curve range: 0.005-4.0 ng/mL
(Adipsin), standard curve range: 0.014-10 ng/mL
(CRP), standard curve range: 0.549-400 ng/mL
(Haptoglobin), standard curve range: 13.717-10,000 ng/mL
(SAP), standard curve range: 6.858-5000 ng/mL
(A2M), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 73-98%, sensitivity: 0.001-0.041 ng/mL
(MinDC+2SD), standard curve range: 0.001-1 ng/mL
(VEGF), standard curve range: 0.005-5 ng/mL
(IP-10), standard curve range: 0.01-15 ng/mL
(KIM-1), standard curve range: 0.02-25 ng/mL
(TIMP-1), standard curve range: 0.05-50 ng/mL
(β-2-Microglobulin), standard curve range: 0.05-50 ng/mL
(Renin)

assay range

accuracy: 77-92%, standard curve range: 12.2-50,000 pg/mL

assay range

accuracy: 84-106%, standard curve range: 137-100,000 pg/mL
(CD40L), standard curve range: 14-10,000 pg/mL
(Endocan-1), standard curve range: 274-200,000 pg/mL
(Follistatin), standard curve range: 34-25,000 pg/mL
(CXCL16, Oncostatin M), standard curve range: 4-3,000 pg/mL
(PIGF-2), standard curve range: 686-500,000 pg/mL
(LIGHT, Troponin I), standard curve range: 69-50,000 pg/mL
(Troponin T), inter-assay cv: <20%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Acute phase proteins are a class of proteins whose circulating concentrations increase (positive acute phase proteins) or decrease (negative acute phase proteins) in response to inflammation. This response is called the acute phase reaction or acute phase response. In recent years several new acute phase proteins have been identified, some of which are secreted from adipose tissue.

MILLIPLEX® Mouse Acute Phase Magnetic Bead Panel 2 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum or plasma samples, mouse tissue extract, or cell/tissue culture supernatant samples: Adipsin (Complement Factor D), Alpha-1 Acid Glycoprotein (AGP), C-Reactive Protein (CRP), Haptoglobin (HPTGN), Alpha-2 Macroglobulin (A2M) and Serum Amyloid Protein/Pentraxin-2 (SAP). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Specificity

Cross Reactivty
Antibody pairs in the panel are specific and demonstrate no cross-reactivity (<0.1%) with
other analytes in the panel.

Application

  • Analytes: α-1, Acid Glycoprotein (AGP), α-2 Macroglobulin (A2M), Adipsin (Factor D), C-Reactive Protein (CRP), Haptoglobin, Serum Amyloid P (SAP)
  • Recommended Sample Type: Mouse serum, plasma or cell/tissue culture supernatants lysates
  • Recommended Sample Dilution: 25 μL per well of 1:20,000 diluted serum or plasma; cell/tissue culture samples may require dilution in appropriate control medium
  • Assay Run Time: Overnight (16-22 hours) at 2-8°C
  • Research Category: Cardiovascular
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


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R B Kostogrys et al.
The journal of nutrition, health & aging, 19(7), 710-718 (2015-07-22)
Low Carbohydrate High Protein diet represents a popular strategy to achieve weight loss. The aim of this study was to characterize effects of low carbohydrate, high protein diet (LCHP) on atherosclerotic plaque development in brachiocephalic artery (BCA) in apoE/LDLR-/- mice
Edward O List et al.
Molecular endocrinology (Baltimore, Md.), 27(3), 524-535 (2013-01-26)
GH receptor (GHR) gene-disrupted mice (GHR-/-) have provided countless discoveries as to the numerous actions of GH. Many of these discoveries highlight the importance of GH in adipose tissue. For example GHR-/- mice are insulin sensitive yet obese with preferential
Marnix Lameijer et al.
Nature biomedical engineering, 2(5), 279-292 (2019-04-03)
Macrophage accumulation in atherosclerosis is directly linked to the destabilization and rupture of plaque, causing acute atherothrombotic events. Circulating monocytes enter the plaque and differentiate into macrophages, where they are activated by CD4+ T lymphocytes through CD40-CD40 ligand signalling. Here
Sarah M Snyder et al.
The Journal of nutrition, 146(5), 1001-1007 (2016-04-08)
Intake of polyphenols and polyphenol-rich fruit extracts has been shown to reduce markers of inflammation, diabetes, and hepatic complications that result from the consumption of a high-fat (HF) diet. The objective of this study was to determine whether mice fed
Nigel Davies et al.
Molecular therapy. Nucleic acids, 24, 369-384 (2021-04-20)
Lipid nanoparticles (LNPs) are the most clinically advanced delivery system for RNA-based drugs but have predominantly been investigated for intravenous and intramuscular administration. Subcutaneous administration opens the possibility of patient self-administration and hence long-term chronic treatment that could enable messenger

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