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MCD8MAG48KPX15BK

Millipore

MILLIPLEX® Mouse CD8 + T Cell Magnetic Bead Panel Premixed Kit

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple cytokine and chemokine CD8+ biomarkers in mouse serum, plasma and cell culture samples.

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eCl@ss:
32161000

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

standard curve range: 12.2-50,000 pg/mL
(IL-6)

standard curve range: 2.4-10,000 pg/mL
(Granzyme B, IL-4, TNFα)

standard curve range: 24.4-100,000 pg/mL
(sFas, sFas Ligand, GM-CSF)

standard curve range: 3.7-15,000 pg/mL
(IL-5)

standard curve range: 4.9-20,000 pg/mL
(sCD137 IFNγ, IL-2, MIP-1ß, RANTES)

standard curve range: 48.8-200,000 pg/mL
(IL-13)

standard curve range: 6.1-25,000 pg/mL
(IL-10)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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HCD8MAG15K17PMXHCD8MAG15KPX17BKMT17MAG47PMX25BK
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

standard curve range: 12.2-50,000 pg/mL
(IL-6), standard curve range: 2.4-10,000 pg/mL
(Granzyme B, IL-4, TNFα), standard curve range: 24.4-100,000 pg/mL
(sFas, sFas Ligand, GM-CSF), standard curve range: 3.7-15,000 pg/mL
(IL-5), standard curve range: 4.9-20,000 pg/mL
(sCD137 IFNγ, IL-2, MIP-1ß, RANTES), standard curve range: 48.8-200,000 pg/mL
(IL-13), standard curve range: 6.1-25,000 pg/mL
(IL-10)

assay range

standard curve range: 0.4-1,500 pg/mL
(IL-6), standard curve range: 0.5-2,000 pg/mL
(TNFα), standard curve range: 1-3,500 pg/mL
(MIP-1α), standard curve range: 1-5,000 pg/mL
(Granzyme B), standard curve range: 1-5,000 pg/mL
(IFNγ), standard curve range: 1-5,000 pg/mL
(IL-5), standard curve range: 10-50,000 pg/mL
(Perforin), standard curve range: 10-50,000 pg/mL
(sFasL), standard curve range: 2-10,000 pg/mL
(IL-4), standard curve range: 2-10,000 pg/mL
(sCD137), standard curve range: 2-7,500 pg/mL
(IL-13), standard curve range: 2-7,500 pg/mL
(IL-2), standard curve range: 20-100,000 pg/mL
(Granzyme A), standard curve range: 4-15,000 pg/mL
(GM-CSF), standard curve range: 400-1,650,000 pg/mL
(sFas), standard curve range: 5-20,000 pg/mL
(IL-10), standard curve range: 7-30,000 pg/mL
(MIP-1β)

assay range

standard curve range: 0.4-1,500 pg/mL
(IL-6), standard curve range: 0.5-2,000 pg/mL
(TNFα), standard curve range: 1-3,500 pg/mL
(MIP-1α), standard curve range: 1-5,000 pg/mL
(Granzyme B), standard curve range: 1-5,000 pg/mL
(IFNγ), standard curve range: 1-5,000 pg/mL
(IL-5), standard curve range: 10-50,000 pg/mL
(Perforin), standard curve range: 10-50,000 pg/mL
(sFasL), standard curve range: 2-10,000 pg/mL
(IL-4), standard curve range: 2-10,000 pg/mL
(sCD137), standard curve range: 2-7,500 pg/mL
(IL-13), standard curve range: 2-7,500 pg/mL
(IL-2), standard curve range: 20-100,000 pg/mL
(Granzyme A), standard curve range: 4-15,000 pg/mL
(GM-CSF), standard curve range: 400-1,650,000 pg/mL
(sFas), standard curve range: 5-20,000 pg/mL
(IL-10), standard curve range: 7-30,000 pg/mL
(MIP-1β)

assay range

-

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

compatibility

configured for Premixed

compatibility

configured for Premixed

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

General description

CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.

The MILLIPLEX® Mouse CD8+ T Cell Bead Panel enables you to focus on the therapeutic potential of Mouse CD8+ T Cell analytes. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX® Mouse CD8+ T Cell Bead Panel is the most versatile system available for Mouse CD8+ T Cell research.

This MILLIPLEX® kit offers you the ability to:
  • Select a 15-plex premixed option or
  • Choose any combination of analytes from our panel of 15 analytes to design a custom kit that better meets your needs.
  • A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.


Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol)..
UPDATE >>
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: sCD137, sFas, sFAS ligand, GM-CSF, Granzyme B, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1β, RANTES, TNF-α
  • Recommended Sample type: serum, plasma and cell culture samples
  • Recommended Sample dilution: neat
  • Assay Run Time: one day or overnight
  • Research Category: Inflammation & Immunology

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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