MCVD2MAG-77K

MILLIPLEX^® Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

• eCl@ss: 32161000

Properties

• description: The MILLIPLEX^® MAP Mouse Cardiovascular Disease Panel 2 is used for the simultaneous quantification of any of the following analytes in serum, plasma, or culture supernatant samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I, Troponin T.
• Quality Level: 200
• species reactivity: mouse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 84-106%; standard curve range: 137-100,000 pg/mL; (CD40L); standard curve range: 14-10,000 pg/mL; (Endocan-1); standard curve range: 274-200,000 pg/mL; (Follistatin); standard curve range: 34-25,000 pg/mL; (CXCL16, Oncostatin M); standard curve range: 4-3,000 pg/mL; (PIGF-2); standard curve range: 686-500,000 pg/mL; (LIGHT, Troponin I); standard curve range: 69-50,000 pg/mL; (Troponin T); inter-assay cv: <20%; intra-assay cv: <10%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Cardiovascular disease (CVD), particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk
factors.

MILLIPLEX^® Mouse CVD Panel 2 is a 9 plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, or tissue culture samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I and Troponin T. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: sCD40L, CXCL16, Endocan-1 (ESM-1), Follistatin, LIGHT, Oncostatin M (OSM), , Placental Growth Factor 2 (PIGF-2), Troponin I (TnI), Troponin T (TnT)
• Recommended Sample Type: Mouse serum, plasma, or cell/tissue culture supernatants or lysates
• Recommended Sample Dilution: 10 μL per well of undiluted serum or plasma; cell/tissue culture supernatants may require dilution in appropriate control medium
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Cardiovascular Disease
• Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Mouse CVD Panel 2 Standard, 1 vial, lyophilized

Mouse CVD Panel 2 Quality Control 1, 1 vial, lyophilized

Mouse CVD Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 bottle, 4 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse CVD Panel 2 Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332 - H311 - H317 - H319 - H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3