MilliporeSigma
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MECY2MAG-73K

Millipore

MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73K

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

eCl@ss:
32161000


To order a Milliplex® kit, please search for your analyte of interest.


Design And Price Your Kit

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 77-105%
standard curve range: 1-5,000 pg/mL
(IFNγ)

standard curve range: 12-50,000 pg/mL
(Exodus-2/CCL21/6Ckine)

standard curve range: 12-50,000 pg/mL
(IFNβ1)

standard curve range: 12-50,000 pg/mL
(IL-11)

standard curve range: 12-50,000 pg/mL
(IL-20)

standard curve range: 12-50,000 pg/mL
(MIP-3β/CCL19)

standard curve range: 2-10,000 pg/mL
(IL-16)

standard curve range: 2-10,000 pg/mL
(MCP-5/CCL12)

standard curve range: 2-10,000 pg/mL
(MDC/CCL22)

standard curve range: 2-10,000 pg/mL
(MIP-3α/CCL20)

standard curve range: 2-10,000 pg/mL
(TARC/CCL17)

standard curve range: 2-10,000 pg/mL
(TIMP-1)

standard curve range: 24-100,000 pg/mL
(Fractalkine/CX3CL1)

standard curve range: 24-100,000 pg/mL
(IL-17A/F)

standard curve range: 5-20,000 pg/mL
(Erythropoietin (EPO))

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition, the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states.

The MILLIPLEX® Mouse Cytokine/Chemokine Bead Panel II, contains all the components necessary to measure the following 15 biomarkers in any combination using Luminex® xMAP® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17, TIMP-1. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate.

Panel Type: Cytokines/Chemokines

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

  • Analytes: Erythropoietin (EPO), Exodus-2, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC (CCL22), MIP-3α/CCL20, MIP-3β/CCL19, TARC/CCL17, TIMP-1
  • Recommended Sample type: serum, plasma, and cell culture
  • Recommended Sample dilution: 1:2 dilution of serum and plasma. Tissue culture supernatant may require a dilution with an appropriate control medium
  • Assay Run Time: overnight or one day
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL

Mouse Cytokine/Chemokine Panel II Antibody-Immobilized Beads (50X), 90 µL ea

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.
Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Joan Antoni Fernández-Blanco et al.
JCI insight, 3(17) (2018-09-07)
The respiratory tract is normally kept essentially free of bacteria by cilia-mediated mucus transport, but in chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF), bacteria and mucus accumulates instead. To address the mechanisms behind the mucus accumulation, the proteome
Susana Rodriguez et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2657-2676 (2020-01-08)
Local and systemic factors that influence renal structure and function in aging are not well understood. The secretory protein C1q/TNF-related protein 1 (CTRP1) regulates systemic metabolism and cardiovascular function. We provide evidence here that CTRP1 also modulates renal physiology in
Susana Rodriguez et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 320(1), R19-R35 (2020-10-22)
C1q/TNF-related protein 1 (CTRP1) is an endocrine factor with metabolic, cardiovascular, and renal functions. We previously showed that aged Ctrp1-knockout (KO) mice fed a control low-fat diet develop renal hypertrophy and dysfunction. Since aging and obesity adversely affect various organ

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