Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition, the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states.
The MILLIPLEX® Mouse Cytokine/Chemokine Bead Panel II, contains all the components necessary to measure the following 15 biomarkers in any combination using Luminex® xMAP® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17, TIMP-1. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate.
Panel Type: Cytokines/Chemokines
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
- Analytes: Erythropoietin (EPO), Exodus-2, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC (CCL22), MIP-3α/CCL20, MIP-3β/CCL19, TARC/CCL17, TIMP-1
- Recommended Sample type: serum, plasma, and cell culture
- Recommended Sample dilution: 1:2 dilution of serum and plasma. Tissue culture supernatant may require a dilution with an appropriate control medium
- Assay Run Time: overnight or one day
- Research Category: Inflammation & Immunology
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Everything you need in a single kit.
Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized
Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized
Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized
Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL
Mouse Cytokine/Chemokine Panel II Antibody-Immobilized Beads (50X), 90 µL ea
Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.
Please contact Technical Service for linearity of dilution.
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MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.