MGAMMAG-300K

MILLIPLEX^® Mouse Immunoglobulin Isotyping Panel

Configurable Mouse Immunoglobulin Isotyping 6-Plex Panel

• Synonym(s): Luminex^® Mouse Isotyping Assay, Millipore Mouse Isotyping Assay, Mouse IgG, IgA, IgM immunoassay, Mouse Isotyoing Multiplex Assay
• UNSPSC Code: 12161503
• NACRES: NA.84
• eCl@ss: 32161000

Properties

• species reactivity: mouse
• Quality Level: 200
• assay range: accuracy: 100%; (IgM), accuracy: 85%; (IgG3), accuracy: 98%; (IgA), accuracy: 98%; (IgG2b), sensitivity: 0.18 ng/mL; (IgG2b), sensitivity: 0.32 ng/mL; (IgG3), sensitivity: 0.67 ng/mL; (IgG2a), sensitivity: 0.7 ng/mL; (IgG1), sensitivity: 1.1 ng/mL; (IgA), sensitivity: 1.73 ng/mL; (IgM), standard curve range: 1-900 ng/mL; (IgG2b), standard curve range: 2-1,500 ng/mL; (IgG1), standard curve range: 2-1,500 ng/mL; (IgG3), standard curve range: 2-1,500 ng/mL; (IgM), standard curve range: 3-2,000 ng/mL; (IgG2a), standard curve range: 5-3,000 ng/mL; (IgA), inter-assay cv: 14-17%; intra-assay cv: 5-10%
• technique(s): multiplexing: suitable
• input: cell culture supernatant(s); tissue/cellular lysate
• packaging: pkg of 1 ea
• manufacturer/tradename: Milliplex^®
• detection method: fluorometric (Luminex^® xMAP^® technology)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes.

Application

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 6 analytes in mouse serum, tissue/cell lysates, and cell culture supernatants.

Analytes included: IgM, IgG1, IgG2a, IgG2b, IgG3, IgA

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

Comprehensive Isotype Profiling: Measures six major mouse immunoglobulin isotypes: IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM—all in a single well.

Flexible Panel Design: Choose only the immunoglobulins relevant to your study, allowing for customized assay configurations.

High Dilution Compatibility: Optimized for 1:50,000 dilution, enabling accurate quantification even in highly concentrated samples.

Fast Assay Turnaround: Complete your assay in one day, streamlining workflows and accelerating data collection.

Support Disease Characterization: Ideal for studying autoimmune disorders, GI conditions, and malignancies where isotype imbalances are key indicators.

Enhance Experimental Efficiency: Multiplex format boosts productivity while conserving sample volume—especially valuable in small animal studies.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302 + H312 + H332,H317,H318,H373,H411
• pcodes: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• target_organs: Respiratory Tract
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3