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MHA000P2D

Millipore

Wallerstein Differential Broth

pkg of 2 mL, ampoule used in breweries to detect and enumerate bacteria present in small numbers in a mixed flora sample. Cycloheximide inhibits most yeasts and molds.

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Synonym(s):
WLD, WL Differential broth
eCl@ss:
32160801
NACRES:
NA.74

Quality Level

form

liquid

shelf life

12 mo.

feature

ready-to-use

packaging

pkg of 2 mL
pack of 50 tests

color

slightly opalescent green-blue

input

sample type sport drink
sample type bottled water
sample type cosmetics
sample type beer
sample type beverage(s)
sample type beverage(s)
sample type soft drink
sample type cider
sample type food(s)
sample type wine

pH

5.5±0.2 (25.0 °C)

application(s)

environmental
food and beverages
industrial qc (microbial monitoring)
microbiology

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

bacteria

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This Item
MHA00P2SMMHA000P2EMHA000P2P
shelf life

12 mo.

shelf life

12 mo.

shelf life

12 mo.

shelf life

12 mo.

feature

ready-to-use

feature

ready-to-use

feature

ready-to-use

feature

ready-to-use

packaging

pkg of 2 mL, pack of 50 tests

packaging

pkg of 2 mL, pack of 50 tests

packaging

ampule of 2 mL, pack of 50 tests

packaging

pkg of 2 mL, pack of 50 tests

pH

5.5±0.2 (25.0 °C)

pH

4.4±0.2 (25.0 °C)

pH

7.2±0.2 (25.0 °C)

pH

7.1±0.2 (25.0 °C)

application(s)

environmental
food and beverages
industrial qc (microbial monitoring)
microbiology

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
microbiology

application(s)

bioburden testing
clinical
cosmetics
environmental
food and beverages
industrial qc
water monitoring, microbiology

application(s)

bioburden testing
clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
microbiology
water monitoring

General description

WL Differential media is recommended for selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations. The presence of cyclohexamide inhibits the growth of yeasts.{4} Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment and trained personnel are often in short supply. They are available in convenient, single test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Application

WL differential media is used in the growth of acetic acid bacteria, Flavobacterium, Proteus and thermophilic bacteria under aerobic and growth of lactic acid bacteria and Pediococcus under anaerobic condition.{5}

Analysis Note

Bacteria appear as small colonies with green blue color. If cycloheximide resistant yeast grow, they are creamy, green-white
QC organisms
A. aceti (ATCC 15973)
Target Microorganisms: Aciduric bacteria (yeast and mold are inhibited)

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Slide 1 of 3

1 of 3

Compendium of methods for the microbiological examination of foods. 2001
American Public Health Association and others
American Public Health Association, 12, 43-43 (2001)
Paper read at American Society of Brewing Chemists Meeting
Wallerstein Laboratories Communications, 12, 43-43 (1950)

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