MHSTCMAG-70K
Synonym(s):
Luminex mouse high sensitivity cytokine panel, Millipore mouse high sensitivity cytokine panel, mouse T cell multiplex panel
MILLIPLEX® Mouse High Sensitivity T Cell Panel,
Configurable Mouse High Sensitivity T Cell 18-Plex Panel
mouse
pkg of 1 ea
Milliplex®
multiplexing: suitable
plasma
cell culture supernatant(s)
serum
accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14.000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%
fluorometric (Luminex® xMAP® technology)
wet ice
2-8°C
1 of 1
This Item | |||
|---|---|---|---|
| species reactivity mouse | species reactivity mouse | species reactivity human | species reactivity mouse |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| assay range accuracy: 82-102%, standard curve range: 0.10-400 pg/mL | assay range accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL | assay range accuracy: 98-107%, sensitivity: 0.11-8.17 pg/mL | assay range accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in ambient | shipped in ambient | shipped in wet ice | shipped in ambient |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) |
Explore all of our products under MILLIPLEX® Mouse High Sensitivity T Cell Panel
Danger
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Respiratory Tract
10 - Combustible liquids
WGK 3
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Find documentation for the products that you have recently purchased in the Document Library.
When running the Mouse High Sensitivity Cytokine Panel with tissue lysates prepared in a Lysis Buffer from BioRad that is compatible with their BioPlex kits, should I use Serum Matrix to prepare the standards, or should I use the buffer in which my samples are prepared?
It is recommended to use the same buffer in which the samples are prepared wherever the protocol specifies the use of Serum Matrix. This ensures consistent assay conditions in the standard, QC, and sample wells.
It's important to note that the kit has not been validated with this buffer, so there is a possibility that some QCs may read out of range when using this buffer. In such cases, it may be necessary to establish their own ranges using this buffer.
Helpful?
Before purchasing the Mouse MHSTCMAG-70K kit, I have a couple of questions regarding the assessment of three cytokines (TNF alpha, IL-6, and IL-1b) in plasma and brain extracts.
I have provided some information that should assist you with your project. There is a protocol available that describes how to create a brain homogenate, including the appropriate concentration. Additionally, I have included a research paper on rat brain with a comprehensive methods section; however, we do not have one available for mouse. You will also find our general guide to buffers. Determining the total protein to add for the assay will depend on the specific experimental conditions. Nevertheless, our cytokine kits offer a wide standard curve range, and considering the sample type, using a more concentrated sample in the assay is likely the best approach. Although we do not use tissue samples in our kits, we have not established the appropriate sample concentration for this. Drawing from our cell signaling kits, which utilize cell lysates, it is recommended to use between 1 and 25 ug of total protein per well in the assay, which may serve as a good starting point for you. In terms of a protease inhibitor, we recommend using the Sigma protease inhibitor cocktail P2714, which should be hydrated in 100 mls of water per bottle and is a 100X stock.
Helpful?
