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MHSTCMAG-70K

MILLIPLEX® Mouse High Sensitivity T Cell Panel

Configurable Mouse High Sensitivity T Cell 18-Plex Panel

Synonym(s):

Luminex mouse high sensitivity cytokine panel, Millipore mouse high sensitivity cytokine panel, mouse T cell multiplex panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000
To order a Milliplex® kit, please search for your analyte of interest.


Product Name

MILLIPLEX® Mouse High Sensitivity T Cell Panel,

Quality Level

description

Configurable Mouse High Sensitivity T Cell 18-Plex Panel

species reactivity

mouse

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

input

plasma
cell culture supernatant(s)
serum

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14.000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

General description

Mouse T cell cytokines are critical signaling molecules produced by T cells that play essential roles in the immune response. They facilitate communication between immune cells, influencing the activation, proliferation, and differentiation of various immune cell types. Key cytokines include interleukins (such as IL-2 and IL-4) and interferons, which help orchestrate both innate and adaptive immune responses in mice.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 18 analytes in mouse serum, plasma, and cell culture supernatants.

Analytes included: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

High Sensitivity Detection: Detects picogram-level cytokine concentrations, ideal for studying subtle immune responses in low-inflammation models.

Broad Analyte Coverage: Measures 18 key cytokines including IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IFNγ, TNFα, GM-CSF, MCP-1, MIP-2, KC, LIX, and IL-7.

Verified for In Vitro and In Vivo Models: Proven performance in stimulated PBMC cultures and mouse models of aging, obesity, and inflammation.

High Precision and Accuracy: Intra-assay CV <10%, inter-assay CV <15%, and accuracy between 82–102% across analytes.

Reveal Hidden Immune Signals: Ideal for detecting low-level cytokine expression that standard assays may miss—critical for understanding early or chronic immune responses.

Legal Information

Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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MHSTCMAG-70KPMXHSTCMAG-28SKMHSTCMAG-70KPXBK
species reactivity

mouse

species reactivity

mouse

species reactivity

human

species reactivity

mouse

assay range

accuracy: 82-102%, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.42-14.000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), inter-assay cv: <15%
intra-assay cv: <10%, standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 3.17-13,000 pg/mL
(LIX)

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(MCP-1/CCL2), standard curve range: 3.42-14,000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), standard curve range: 9.77-40,000 pg/mL
(LIX), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 98-107%, sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC), standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.24-1,000 pg/mL
(IL-21), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.31-1,250 pg/mL
(MIP-1α), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.45-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 0.61-2,500 pg/mL
(MIP-3α), standard curve range: 0.73-3,000 pg/mL
(IL-17A), standard curve range: 0.92-3,750 pg/mL
(MIP-1β), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.46-6,000 pg/mL
(ITAC), standard curve range: 1.83-7,500 pg/mL
(IL-4), standard curve range: 18.3-75,000 pg/mL
(Fractalkine), standard curve range: 7.93-32,500 pg/mL
(IL-23), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14,000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

ambient

shipped in

ambient

shipped in

wet ice

shipped in

ambient


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signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MHSTCMAG-70K-04C04054839442254
MHSTCMAG-70K-09C04054839442308
MHSTCMAG-70K-08C04054839442292
MHSTCMAG-70K-10C04054839442315
MHSTCMAG-70K-15C04054839442360
MHSTCMAG-70K-16C04054839442377
MHSTCMAG-70K-03C04054839442247
MHSTCMAG-70K-05C04054839442261
MHSTCMAG-70K-14C04054839442353
MHSTCMAG-70K-13C04054839442346
MHSTCMAG-70K-18C04054839442407

Questions

1–2 of 2 Questions  
  1. When running the Mouse High Sensitivity Cytokine Panel with tissue lysates prepared in a Lysis Buffer from BioRad that is compatible with their BioPlex kits, should I use Serum Matrix to prepare the standards, or should I use the buffer in which my samples are prepared?

    1 answer
    1. It is recommended to use the same buffer in which the samples are prepared wherever the protocol specifies the use of Serum Matrix. This ensures consistent assay conditions in the standard, QC, and sample wells.

      It's important to note that the kit has not been validated with this buffer, so there is a possibility that some QCs may read out of range when using this buffer. In such cases, it may be necessary to establish their own ranges using this buffer.

      Helpful?

  2. Before purchasing the Mouse MHSTCMAG-70K kit, I have a couple of questions regarding the assessment of three cytokines (TNF alpha, IL-6, and IL-1b) in plasma and brain extracts.

    1 answer
    1. I have provided some information that should assist you with your project. There is a protocol available that describes how to create a brain homogenate, including the appropriate concentration. Additionally, I have included a research paper on rat brain with a comprehensive methods section; however, we do not have one available for mouse. You will also find our general guide to buffers. Determining the total protein to add for the assay will depend on the specific experimental conditions. Nevertheless, our cytokine kits offer a wide standard curve range, and considering the sample type, using a more concentrated sample in the assay is likely the best approach. Although we do not use tissue samples in our kits, we have not established the appropriate sample concentration for this. Drawing from our cell signaling kits, which utilize cell lysates, it is recommended to use between 1 and 25 ug of total protein per well in the assay, which may serve as a good starting point for you. In terms of a protease inhibitor, we recommend using the Sigma protease inhibitor cocktail P2714, which should be hydrated in 100 mls of water per bottle and is a 100X stock.

      Helpful?

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