MilliporeSigma
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MHSTCMAG-70K

Millipore

MILLIPLEX® Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

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Synonym(s):
Luminex® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay
eCl@ss:
32161000

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 82-102%
sensitivity: 0.26-11.14 pg/mL
standard curve range: 0.10-400 pg/mL
(IL-4)

standard curve range: 0.49-2,000 pg/mL
(IFNγ)

standard curve range: 0.49-2,000 pg/mL
(IL-17A)

standard curve range: 0.49-2,000 pg/mL
(IL-7)

standard curve range: 0.49-2,000 pg/mL
(TNFα)

standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1)

standard curve range: 0.98-4,000 pg/mL
(IL-2)

standard curve range: 0.98-4,000 pg/mL
(IL-5)

standard curve range: 0.98-4,000 pg/mL
(IL-6)

standard curve range: 1.34-5,500 pg/mL
(IL-10)

standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70))

standard curve range: 3.17-13,000 pg/mL
(LIX)

standard curve range: 3.42-14.000 pg/mL
(IL-13)

standard curve range: 3.91-16,000 pg/mL
(IL-1α)

standard curve range: 3.91-16,000 pg/mL
(IL-1β)

standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2)

standard curve range: 6.1-25,000 pg/mL
(GM-CSF)

standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2)

inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

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This Item
MHSTCMAG-70KPXBKHSTCMAG384-PX21HCYTOMAG-60K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14.000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14,000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 79-108%, standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.24-1,000 pg/mL
(IL-21), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.31-1,250 pg/mL
(MIP-1α), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.43-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 0.61-2,500 pg/mL
(MIP-3α), standard curve range: 0.73-3,000 pg/mL
(IL-17A), standard curve range: 0.92-3,750 pg/mL
(MIP-1β), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.46-6,000 pg/mL
(ITAC), standard curve range: 1.83-7,500 pg/mL
(IL-4), standard curve range: 18.3-75,000 pg/mL
(Fractalkine), standard curve range: 7.93-32,500 pg/mL
(IL-23), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 87-107%, standard curve range: 3.2-10,000 pg/mL
(sCD40L, EGF, Eotaxin/CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF), inter-assay cv: 5-19%
intra-assay cv: 2-13%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

wet ice

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
There is no detectable cross-reactivity within the panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α
  • Recommended Sample type: serum, plasma, tissue culture or other media
  • Recommended Sample dilution: neat or 1:2 diluted serum or plasma
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Immune Response Metabolic Disorders Chronic Inflammatory Disease Cardiovascular Disease

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see protocol for sensitivity of individual analytes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

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705578-5MG-PW

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MMYOMAG-74K-13

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Anna M Klawonn et al.
Immunity, 54(2), 225-234 (2021-01-22)
Microglia are activated in many neurological diseases and have been suggested to play an important role in the development of affective disorders including major depression. To investigate how microglial signaling regulates mood, we used bidirectional chemogenetic manipulations of microglial activity
Sha-Sha Yi et al.
Frontiers in psychiatry, 13, 864481-864481 (2022-05-17)
Increasing evidence indicates that inflammatory responses may influence brain neurochemical pathways, inducing depressive-like behaviors. Ultrasound stimulation (US) is a promising non-invasive treatment for neuropsychiatric diseases. We investigated whether US can suppress inflammation and improve depressive-like behaviors. Mice were intraperitoneally injected
Robert A Kazmierczak et al.
Oncotarget, 11(44), 3943-3958 (2020-11-21)
Conventional cancer chemotherapies are not fully efficacious and do not target tumors, leading to significant treatment-related morbidities. A number of genetically attenuated cancer-targeting bacteria are being developed to safely target tumors in vivo. Here we report the toxicological, tumor-targeting, and
Sébastien Le Garf et al.
International journal of molecular sciences, 20(20) (2019-10-23)
Regular aerobic exercise, independently of weight loss, improves metabolic and anti-inflammatory states, and can be regarded as beneficial in counteracting obesity-induced low-grade inflammation. However, it is still unknown how exercise alters immunometabolism in a context of dietary changes. Agonists of
David K Harrison et al.
Transplantation, 105(3), 540-549 (2020-09-06)
Organ transplantation is life-saving and continued investigations into immunologic mechanisms that drive organ rejection are needed to improve immunosuppression therapies and prevent graft failure. DNA-dependent protein kinase catalytic subunit, DNA dependent-protein kinase catalytic subunit (DNA-PKcs), is a critical component of

Related Content

Evaluation of MILLIPLEX® Mouse High Sensitivity T Cell Panel

The MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panel is a powerful tool for cytokine profiling in biological samples. Uncover details on how this panel was evaluated for studying low-level cytokine expression and also quantifying multiple cytokine secretion levels simultaneously.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service