The use of deionized water is crucial for immunoassays in general. In this case, lower signal for the standards and controls was observed when non-deionized water was used. Despite the buffers containing EDTA, it seems that they may have been overloaded with ions and thus not able to chelate all of them.
MPTMAG-49K
MILLIPLEX® Mouse Pituitary Panel
Configurable Mouse Pituitary 7-Plex Panel
Synonym(s):
MPTMAG-49K product info, endocrine biomarker multiplex kit, mouse endocrine research tool, mouse growth and metabolism panel, mouse hormone assay overview, mouse pituitary hormone panel, multiplex hormone profiling kit, neuroendocrine marker panel mouse, pituitary function biomarker listing, reproductive biology product sheet
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
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species reactivity
mouse
Quality Level
assay range
accuracy: 96-101%, standard curve range: 12.2-50,000 pg/mL
(TSH), standard curve range: 122-500,000 pg/mL
(Prolactin), standard curve range: 2.4-10,000 pg/mL
(ACTH), standard curve range: 2.4-10,000 pg/mL
(BDNF), standard curve range: 24.4-100,000 pg/mL
(FSH), standard curve range: 4.9-20,000 pg/mL
(GH), standard curve range: 4.9-20,000 pg/mL
(LH), inter-assay cv: <20%
intra-assay cv: <15%
technique(s)
multiplexing: suitable
input
tissue/cellular lysate
plasma
culture supernatant(s)
packaging
pkg of 1 ea
manufacturer/tradename
Milliplex®
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
1 of 4
This Item | RPTMAG-86K | HPTP1MAG-66K | NHPPT1MG-46K |
|---|---|---|---|
| species reactivity mouse | species reactivity rat | species reactivity human | species reactivity nonhuman primates |
| assay range accuracy: 96-101%, standard curve range: 122-500,000 pg/mL | assay range accuracy: 75% | assay range accuracy: 105% | assay range accuracy: 112% |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 7 analytes in mouse serum, plasma, or cell/tissue culture supernatants.
Analytes Included: ACTH, BDNF, FSH, GH, LH, Prolactin, TSH
Assay Characteristics: Refer to assay protocol for details on cross-reactivity, sensitivity, precision, and accuracy.
Analytes Included: ACTH, BDNF, FSH, GH, LH, Prolactin, TSH
Assay Characteristics: Refer to assay protocol for details on cross-reactivity, sensitivity, precision, and accuracy.
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Features and Benefits
Features:
- Targets key pituitary hormones involved in endocrine and neuroendocrine regulation
- Includes 7 analytes relevant to metabolism, growth, reproduction, and neuronal function
- Multiplex format enables simultaneous detection from minimal sample volume
- Compatible with mouse serum, plasma, and cell/tissue culture supernatants
- Designed for research in reproductive biology, endocrine disorders, and neuroendocrine signaling
- Provides a comprehensive profile of pituitary hormone biomarkers in mouse models
- Supports investigation of endocrine function and hormonal regulation mechanisms
- Facilitates evaluation of therapeutic interventions and endocrine toxicity
- Enables high-throughput analysis with reduced sample and reagent use
- Delivers reproducible, sensitive results for preclinical and translational research
General description
The pituitary gland, often referred to as the “master gland,” regulates multiple endocrine functions through the secretion of key hormones. This panel—ACTH, BDNF, FSH, GH, LH, Prolactin, and TSH—captures essential indicators of adrenal, thyroid, gonadal, and neuroendocrine activity. The MILLIPLEX® Mouse Pituitary Panel enables multiplexed quantification of these biomarkers in mouse serum or plasma, supporting research in reproductive biology, metabolism, and endocrine disorders.
Legal Information
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Certificates of Analysis (COA)
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Marina O Fernandez et al.
Endocrinology, 158(1), 121-133 (2016-11-15)
The peroxisome-proliferator activated receptor γ (PPARγ) is expressed in the hypothalamus in areas involved in energy homeostasis and glucose metabolism. In this study, we created a deletion of PPARγ brain-knockout (BKO) in mature neurons in female mice to investigate its
Tomohiro Terasaka et al.
Endocrinology, 160(8), 1999-2014 (2019-06-13)
Gonadotropin secretion, which is elicited by GnRH stimulation of the anterior pituitary gonadotropes, is a critical feature of reproductive control and the maintenance of fertility. In addition, activation of the GnRH receptor (GnRHR) regulates transcription and translation of multiple factors
Thomas Blum et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(30), 15236-15243 (2019-07-10)
Dopamine neurons of the hypothalamic arcuate nucleus (ARC) tonically inhibit the release of the protein hormone prolactin from lactotropic cells in the anterior pituitary gland and thus play a central role in prolactin homeostasis of the body. Prolactin, in turn
Karen J Tonsfeldt et al.
Journal of the Endocrine Society, 3(4), 716-733 (2019-03-25)
In rodents, the preovulatory LH surge is temporally gated, but the timing cue is unknown. Estrogen primes neurons in the anteroventral periventricular nucleus (AVPV) to secrete kisspeptin, which potently activates GnRH neurons to release GnRH, eliciting a surge of LH
Amanda Rodriguez et al.
Development (Cambridge, England), 146(23) (2019-11-11)
The number and quality of oocytes within the ovarian reserve largely determines fertility and reproductive lifespan in mammals. An oocyte-specific transcription factor cascade controls oocyte development, and some of these transcription factors, such as newborn ovary homeobox gene (NOBOX), are
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Was this a case of troubleshooting low MFIs across the standards and controls, with controls falling out of range due to the use of sterile non-deionized water to hydrate the standards and controls?
1 answer-
Helpful?
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In Milliplex Analyst, the concentrations are reported in two columns. One is labeled pg/ml(i) and one is labeled pg/ml. What is the difference between these values?
1 answer-
The pg/ml values represent individual results and are not adjusted for the dilution factor. Additionally, the pg/ml values are the average results of the replicates, and if a dilution factor is present, this value will be multiplied by the dilution factor.
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