MPTMAG-49K

MILLIPLEX^® Mouse Pituitary Magnetic Bead Panel - Endocrine Multiplex Assay

The analytes available for this multiplex kit are: ACTH, BDNF, FSH, GH, Prolactin, TSH, LH.

• eCl@ss: 32161000

Properties

• Quality Level: 200
• species reactivity: mouse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 96-101%; standard curve range: 12.2-50,000 pg/mL; (TSH); standard curve range: 122-500,000 pg/mL; (Prolactin); standard curve range: 2.4-10,000 pg/mL; (ACTH); standard curve range: 2.4-10,000 pg/mL; (BDNF); standard curve range: 24.4-100,000 pg/mL; (FSH); standard curve range: 4.9-20,000 pg/mL; (GH); standard curve range: 4.9-20,000 pg/mL; (LH); inter-assay cv: <20%; intra-assay cv: <15%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Called the “master gland” because it controls many other endocrine glands, the pituitary secretes several key hormones that play important roles in the regulation of metabolism, growth, and reproduction. Piuitary hormones are responsible for the stimulation of the adrenal gland (ACTH), thyroid gland (TSH), ovaries and testes (FSH, LH) and breast milk production (prolactin), as well as blood pressure and the amount of water excreted by the kidneys. Through its connection with the pituitary, the hypothalamus modulates endocrine function by detecting hormone levels. Brain-Derived Neurotrophic Factor (BDNF) is a member of NGF family of neurotrophic factors which are required for differentiation and survival of specific neuronal sub-populations.

MILLIPLEX^® Mouse Pituitary Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum, plasma, and tissue culture samples: ACTH, BDNF, FSH, GH, Prolactin, TSH and LH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Application

• Analytes: ACTH, BDNF, FSH, GH, LH, Prolactin, TSH
• Recommended Sample Type: Mouse serum, plasma, cell/tissue culture supernatants or lysates
• Recommended Sample Dilution: 10 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
• Assay Run Time: For greater sensitivity an overnight incubation is recommended, (16-18 hours) at 2-8°C. Alternatively, incubate for 2 hours at room temperature (20-25°C).
• Research Category: Endocrine
• Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Sensitivity: Please see kit protocol for sensitive’s of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332 - H311 - H315 - H317 - H319 - H335 - H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3